Regional antimicrobial resistance gene flow among the One Health sectors in China.

Background: Antimicrobial resistance poses a significant threat to global health, with its spread intricately linked across human, animal, and environmental sectors. Revealing the antimicrobial resistance gene (ARG) flow among the One Health sectors is essential for better control of antimicrobial resistance.

Results: In this study, we investigated regional ARG transmission among humans, food, and the environment in Dengfeng, Henan Province, China by combining large-scale metagenomic sequencing with culturing of resistant bacterial isolates in 592 samples.

The sow vaginal and gut microbiota associated with longevity and reproductive performance.

Background: Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on the genetic and genomic factors for selection, little is known about the associations between the microbiome and sows with longevity in reproduction.

Results: In this study, we collected and sequenced rectal and vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity.

Effect of PCSK9 inhibitor on early neurological deterioration in acute ischemic stroke patients with a history of coronary heart disease: a study protocol for a randomized controlled trial in Dalian, China.

Background: Early neurological deterioration (END) is a critical determinant influencing the short-term prognosis of acute ischemic stroke (AIS) patients and is associated with increased mortality rates among hospitalized individuals. AIS frequently coexists with coronary heart disease (CHD), complicating treatment and leading to more severe symptoms and worse outcomes. Shared risk factors between CHD and AIS, especially elevated low-density lipoprotein cholesterol (LDL-C), contribute to atherosclerosis and inflammation, which worsen brain tissue damage.

Association between the changes in the estimated glucose disposal rate and new-onset cardiovascular disease in middle-aged and elderly individuals: A nationwide prospective cohort study in China.

Aims: Previous studies have shown that eGDR and TyG, as indicators of insulin resistance (IR), were key risk factors for cardiovascular disease (CVD). Our study further explored the relationship between eGDR change and new-onset CVD, and compared the predictive value of eGDR change, eGDR and TyG.

Materials And Methods: A total of 2895 participants without CVD at baseline from the China Health and Retirement Longitudinal Study (CHARLS) were included, using K-means clustering and cumulative eGDR to measure eGDR change between 2012 and 2015.

Crosstalk between ferroptosis and autophagy: broaden horizons of cancer therapy.

Ferroptosis and autophagy are two main forms of regulated cell death (RCD). Ferroptosis is a newly identified RCD driven by iron accumulation and lipid peroxidation. Autophagy is a self-degradation system through membrane rearrangement.

Factors influencing the type of self-acceptance in drug addicts and the relationship with subthreshold depression: evidence based on latent profile analysis.

Objective: To study the heterogeneity and influencing factors of self-acceptance of drug addicts in compulsory isolation in 2 drug rehabilitation centers in Sichuan Province, and to analyze the effects of different types of self-acceptance on subthreshold depression in drug addicts, in order to provide useful references for improving their mental health.

Methods: Self-acceptance Questionnaire (SAQ), Drug use Stereotype Threat Scale(DSTS) and Center for Epidemiologic Studies Depression Scale(CES-D) were used to conduct a questionnaire survey in January 2024 on 1068 drug addicts in two compulsory isolation drug rehabilitation centers in Sichuan Province.

Results: Drug addicts self-acceptance can be categorized into 5 potential categories; men were more likely than women to show lower self-acceptance in the 'low-low'(β = 0.

Whole-genome sequencing identifies novel loci for keratoconus and facilitates risk stratification in a Han Chinese population.

Background: Keratoconus (KC) is a prevalent corneal condition with a modest genetic basis. Recent studies have reported significant genetic associations in multi-ethnic cohorts. However, the situation in the Chinese population remains unknown.

Periostin-mediated NOTCH1 activation between tumor cells and HSCs crosstalk promotes liver metastasis of small cell lung cancer.

Background: Metastasis is the primary cause of mortality in small cell lung cancer (SCLC), with the liver being a predominant site for distal metastasis. Despite this clinical significance, mechanisms underlying the interaction between SCLC and liver microenvironment, fostering metastasis, remain unclear.

Methods: SCLC patient tissue array, bioinformatics analysis were performed to demonstrate the role of periostin (POSTN) in SCLC progression, metastasis, and prognosis.

The additive effect of the stress hyperglycemia ratio on type 2 diabetes: a population-based cohort study.

Background: The stress hyperglycemia ratio (SHR) has recently gained attention as a marker for acute hyperglycemia, which has been linked to adverse clinical outcomes. However, its independent role in the development of type 2 diabetes (T2D) remains understudied. This cohort study aimed to assess the association between SHR and the incidence of T2D.

Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits.

Background: FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. This study compared the effectiveness of seven different commercially available RNA extraction kits specifically designed for use with FFPE samples in terms of the quantity and quality of RNA recovered.

Methods: This study used 9 samples of FFPE tissue from three different types of tissue (Tonsil, Appendix and lymph node of B-cell lymphoma) to evaluate RNA extraction methods.

Pathogenicity of tick-derived lymphocytic choriomeningitis virus in BALB/c mice.

Background: Lymphocytic choriomeningitis virus (LCMV) is a zoonotic pathogen primarily transmitted by rodents. Recently, LCMV has been detected in ticks from northeastern China; however, the pathogenicity of this virus in murine models remains to be elucidated.

Results: Here, we examined the tick-derived LCMV strain JX14 by inoculating BALB/c mice with 3.

Single-cell RNA sequencing highlights the unique tumor microenvironment of small cell neuroendocrine cervical carcinoma.

Small cell neuroendocrine cervical carcinoma is a highly aggressive tumor characterized by early metastasis, a high recurrence rate, and poor prognosis. This study represents the first instance of single-cell sequencing conducted on small cell neuroendocrine carcinoma of the cervix worldwide. Analysis of gene expression regulatory networks revealed that the transcription factor TFF3 drived up-regulation of ELF3.

Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity.

Background: Drug resistance and immune escape continue to contribute to poor prognosis in AML. Increasing evidence suggests that exosomes play a crucial role in AML immune microenvironment.

Methods: Sanger sequencing, RNase R and fluorescence in situ hybridization were performed to confirm the existence of circ_0006896.

NLRP3 inflammasome-modulated angiogenic function of EPC via PI3K/ Akt/mTOR pathway in diabetic myocardial infarction.

Background: Inflammatory diseases impair the reparative properties of endothelial progenitor cells (EPC); however, the involvement of diabetes in EPC dysfunction associated with myocardial infarction (MI) remains unknown.

Methods: A model was established combining high-fat diet (HFD)/streptozotocin (STZ)-induced diabetic mice with myocardial infarction. The therapeutic effects of transplanted wild-type EPC, Nlrp3 knockout EPC, and Nlrp3 overexpression EPC were evaluated.

Psychological stress and depression symptoms in nursing undergraduates: the chain mediating effect of cognitive reappraisal and ruminate thinking.

Background: Nursing students exhibit a higher incidence of mental disorders. Studies have identified psychological stress contributes to elevated depression symptoms through reappraisal cognitive in nursing students. However, there is little research exploring the knowledge regarding the role of ruminative thinking in mediating the associations between psychological stress and depression symptoms.

Elbow rotation affects the accuracy of rotational formulas: validation of a modified method.

Background: Supracondylar humerus fractures (SCHFs) are the most common elbow fractures in children and are typically treated with closed reduction and Kirschner pin fixation. However, varying degrees of residual rotational displacement may remain after closed reduction. Several methods exist to assess rotational displacement, but none account for the effect of elbow rotation on the results.

Comparative study of torque expression and its biomechanical effects: spherical self-ligating bracket with lock-hook system versus passive self-ligating bracket and conventional bracket.

Background: Proper torque control is crucial to the outcome of orthodontic treatment. This study aimed to employ finite element analysis to compare the torque capabilities of a novel spherical self-ligating bracket with a lock-hook system against those of commonly used passive self-ligating and conventional bracket systems, as well as to reveal the biomechanical changes in the periodontal ligament (PDL) during torque expression.

Methods: A maxillary right central incisor, along with its PDL and alveolar bone, were modeled.

Identification of macrophage polarisation and mitochondria-related biomarkers in diabetic retinopathy.

Background: The activation of macrophages or microglia in patients' whole body or local eyes play significant roles in diabetic retinopathy (DR). Mitochondrial function regulates the inflammatory polarization of macrophages. Therefore, the common mechanism of mitochondrial related genes (MRGs) and macrophage polarisation related genes (MPRGs) in DR is explored in our study to illustrate the pathophysiology of DR.

Association of genetically proxied cancer-targeted drugs with cardiovascular diseases through Mendelian randomization analysis.

Background: Cancer-targeted therapies are progressively pivotal in oncological care. Observational studies underscore the emergence of cancer therapy-related cardiovascular toxicity (CTR-CVT), impacting patient outcomes. We aimed to investigate the causal relationship between different types of cancer-targeted therapies and cardiovascular disease (CVD) outcomes through a two-sample Mendelian randomization (MR) study.

Cromolyn sodium reduces LPS-induced pulmonary fibrosis by inhibiting the EMT process enhanced by MC-derived IL-13.

Background: Sepsis is a systemic inflammatory response caused by infection. When this inflammatory response spreads to the lungs, it can lead to acute lung injury (ALI) or more severe acute respiratory distress syndrome (ARDS). Pulmonary fibrosis is a potential complication of these conditions, and the early occurrence of pulmonary fibrosis is associated with a higher mortality rate.

LIN28A-dependent lncRNA NEAT1 aggravates sepsis-induced acute respiratory distress syndrome through destabilizing ACE2 mRNA by RNA methylation.

Background: Acute respiratory distress syndrome (ARDS) is a life-threatening and heterogeneous disorder leading to lung injury. To date, effective therapies for ARDS remain limited. Sepsis is a frequent inducer of ARDS.

Helicobacter pylori infection promotes M1 macrophage polarization and gastric inflammation by activation of NLRP3 inflammasome via TNF/TNFR1 axis.

Background: Macrophages play a crucial role in chronic gastritis induced by the pathogenic Helicobacter pylori (H. pylori) infection. NLRP3 inflammasome has emerged as an important component of inflammatory processes.

A study on the professional identity intervention of Chinese new nurses by Balint groups from the perspective of ABC theory of emotion-hong.

Objective: To explore the intervention effect of ABC theory on the professional identity and irrational beliefs of new Chinese nurses in Balint groups (BGs).

Methods: A total of 110 Chinese nurses newly recruited in August 2021 by a grade-A tertiary general hospital in Wenzhou, Zhejiang province were adopted as research objects. They were randomly divided into an experimental group and a control group, the experimental group spread out in groups to practice the BGs, and the control group did not have any intervention.

Association between lipid accumulation product and chronic obstructive pulmonary disease: a cross-sectional analysis.

Background: Research indicates that obesity can worsen the clinical manifestations of chronic obstructive pulmonary disease (COPD). Timely detection of COPD has the potential to enhance treatment results. This study seeks to investigate the association between a new metabolic indicator, the lipid accumulation product (LAP), and the risk of developing COPD.

Factors influencing changes in the quality of life of the Hainan migratory population with hypertension: a survey of the Chengmai mangrove bay community.

Background: Hainan is a tropical island in China with a large migratory population. Study have reported that the blood pressure of Hainan elderly hypertensive migratory population decreased significantly, which may be related to the improvement of environment and quality of life (QoL). Understanding the changes of QoL of these people before and after coming to Hainan and its influencing factors can provide a basis for the prevention and control of hypertension.