ML216-Induced BLM Helicase Inhibition Sensitizes PCa Cells to the DNA-Crosslinking Agent Cisplatin.

Using standard DNA-damaging medicines with DNA repair inhibitors is a promising anticancer tool to achieve better therapeutic responses and reduce therapy-related side effects. Cell viability assay, neutral comet assay, western blotting (WB), and cell cycle and apoptosis analysis were used to determine the synergistic effect and mechanism of ML216, a Bloom syndrome protein (BLM) helicase inhibitor, and cisplatin (CDDP), a DNA-crosslinking agent, in PCa cells. Based on the online database research, our findings revealed that BLM was substantially expressed in PCa, which is associated with a bad prognosis for PCa patients.

[Location, selection, and comparative anatomy of "Taixi" (KI3), "Shuiquan" (KI5), "Fuliu" (KI7), "Jiaoxin" (KI8), "Zhubin" (KI9), and "Yingu"(KI10) acupoints in rabbits].

Objective: To investigate the.

Methods: for locating and selecting the acupoints of "Taixi" (KI3), "Shuiquan" (KI5), "Fuliu" (KI7), "Jiaoxin" (KI8), "Zhubin" (KI9), and "Yingu" (KI10) and the morphological structure of these acupoints in rabbits. MethodsAccording to the WHO and national standards for human acupoints and rabbit X-ray images, acupoint locations were marked using the anatomical landmarks on body surface in 10 New Zealand rabbits.

[Hierarchical structure of Five-shu points and Source-point of Triple Energizer Meridian in rabbits].

Objective: To study the position and hierarchical structure of Five-shu points, i．e., "Guanchong" (TE1), "Yemen" (TE2), "Zhongzhu" (TE3), "Yangchi" (TE4) and "Zhigou" (TE6), and the Source-point "Tianjing" (TE10) of the Triple Energizer (TE) Meridian in the rabbit.

Methods: Based on WHO Standard Acupuncture Point Locations in the Western Pacific Region (WHO Standard) and National Standard (GB/T 22103-2008) for Acupuncture Point Locations in human body, and combined with X-ray images, the hierarchical structure of Five-shu points and Source-point of the TE Meridian were observed in ten New Zealand rabbits.

Endothelial-Dependent and Independent Vascular Relaxation Effect of Tetrahydropalmatine on Rat Aorta.

Tetrahydropalmatine (THP) is an active natural alkaloid isolated from W.T. Wang which has been widely used for treating pain and cardiovascular disease in traditional Chinese medicine.

Schisantherin A protects against 6-OHDA-induced dopaminergic neuron damage in zebrafish and cytotoxicity in SH-SY5Y cells through the ROS/NO and AKT/GSK3β pathways.

Ethnopharmacological Relevance: The fruit of Schisandra chinensis (Turcz.) Baill, has been traditionally used in management of liver diseases and ageing associated neurodegeneration. The bioactive compound from this medicinal plant would be valuable for its potential use in prevention and treatment of Parkinson׳s disease.

Discovery of novel anti-parkinsonian effect of schisantherin A in in vitro and in vivo.

Dibenzocyclooctadiene lignans represent a unique group of natural chemical structures, are considered as protectants against neuronal cell death and cognitive impairment in neurological disorders. Among the family of dibenzocyclooctadiene lignan analogs from the fruit of Schisandra chinensis (Turcz.) Baill, neuroprotective potential of schisantherin A (StA) has not yet been characterized.

Discovery of a benzofuran derivative (MBPTA) as a novel ROCK inhibitor that protects against MPP⁺-induced oxidative stress and cell death in SH-SY5Y cells.

Parkinson disease (PD) is a neurodegenerative disease with multifactorial etiopathogenesis. The discovery of drug candidates that act on new targets of PD is required to address the varied pathological aspects and modify the disease process. In this study, a small compound, 2-(5-methyl-1-benzofuran-3-yl)-N-(5-propylsulfanyl-1,3,4-thiadiazol-2-yl) acetamide (MBPTA) was identified as a novel Rho-associated protein kinase inhibitor with significant protective effects against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced damage in SH-SY5Y neuroblastoma cells.

Danshensu protects against 6-hydroxydopamine-induced damage of PC12 cells in vitro and dopaminergic neurons in zebrafish.

The overproduction of reactive oxygen species (ROS) has been implicated in the development of neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD). Previous studies have indicated that danshensu (beta-3,4-dihydroxyphenyl-lactic acid), a main hydrophilic component of the Chinese materia medica Radix Salviae Miltiorrhizae (Danshen, Pharmacopoeia of PR China), has ROS scavenging and antioxidant activities, however its mechanism of action was not clear. In this study, we investigated whether the protective effects of danshensu against neurotoxin 6-hydroxydopamine (6-OHDA)-induced oxidative stress involved the Nrf2/HO-1 pathways.

[Use of zebrafish models for the research of traditional Chinese medicine].

Traditional Chinese medicine (TCM) exhibits a broad range of effects on biological activity that is probably due to interactions of complex chemical constituents with multiple targets in the body. Understanding the active chemical constituents in TCM is very important in providing rationales for the clinical usage of TCM. The zebrafish (Danio rerio) has recently become a popular model in the field of drug screening, specifically emerging as an important vertebrate model for in vivo high-content drug screening of multiple efficacy parameters and whole-organism toxicity.

[Macroscopical anatomic structure of acupoint "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4), and "Quchi" (LI 11) regions in the forelimb in rabbits].

Objective: To observe the structure of acupoints: "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4) and "Ouchi" (LI 11) of the Yangming Meridian in the rabbit's forelimb.

Methods: The acupoints were first located in accordance with the related atlas of rabbits and confirmed by low resistance and higher electric potential determinations by using a "multipurpose electronic acupoint detection and therapeutic instrument". Then the animals under anesthesia were killed by intravenous injection of air embolism, perfused with 5% natrium citricum first, acetic ether containing acrylonitrile-butadiene-styrene copolymer, and 5% dicapryl phthalate, blue and red pigments for paintings via subclavian artery, respectively.