[Zymohydrolysis with chondroitinase ABC and high performance liquid chromatography used for the determination of hyaluronic acid in shark fin].

A method for the determination of hyaluronic acid in shark fin by high performance liquid chromatography (HPLC) is described. At 37 degrees C, with 0.2 mol/L Tris-HCl buffer solution, hyaluronic acid was converted to the hyaluronic acid disaccharide by zymohydrolysis with chondroitinase ABC.