Origin and de novo domestication of sweet orange.

Sweet orange is cultivated worldwide but suffers from various devastating diseases because of its monogenetic background. The elucidation of the origin of a crop facilitates the domestication of new crops that may better cope with new challenges. Here we collected and sequenced 226 citrus accessions and assembled telomere-to-telomere phased diploid genomes of sweet orange and sour orange.

Laparoscopic Extended Segmentectomy VII Guided by the Right Hepatic Vein: Precise Surgical Planning with a Three-Dimensional Liver Model.

Background: Laparoscopic extended segmentectomy VII is a technically challenging procedure owing to a lack of clear anatomical landmarks and difficulty in determining the cutting plane (Wang in J Am Coll Surg 238:321-330, 2024; Liu in Surg Oncol 38:101575, 2021). On the basis of precise surgical planning, we present a laparoscopic extended segmentectomy VII guided by the right hepatic vein.

Patient And Methods: A 65 year-old male patient presented with a right hepatic mass.

Influence of coordination number and ionic radius on metal ion preference and activity of lanthanide-dependent alcohol dehydrogenase: Insights from mutational studies and density functional theory.

Lanthanide (Ln) elements form a cofactor complex with pyrroloquinoline quinone (PQQ) in bacterial alcohol dehydrogenases (Ln-ADH). The lanthanide elements did not support Ln-ADH activity equally, with only early lanthanides (La-Gd) promoting high enzyme activity. However, the early lanthanides did not promote the activity equally and the detailed mechanism of Ln-ADH exhibiting different activity in the presence of different light Lns remains obscure.

Nurses' Knowledge in the Prevention and Management of Nasointestinal Tube Obstruction: A Cross Sectional Study.

Background: The nasointestinal tube is a route of nutritional support for patients and maintaining patency is essential. Given the key role nurses play in the management and monitoring of these tubes, they need to have sufficient knowledge and skills to ensure the safety and well-being of the patients.

Objective: To investigate the status quo of nurses' knowledge of prevention and management of nasointestinal tube obstruction in six hospitals, and to analyze the influencing factors.

Geomimetic Interfacial Hydrothermal Synthesis of Crystalline Ionic Vinylene-Linked Covalent Organic Frameworks.

Ionic vinylene-linked covalent organic frameworks (ivCOFs) with ionic characteristics and highly conjugated structures have been promising functional materials. However, both the number of reported ionic vinylene-linked COFs and its synthetic methodologies still be limited. Herein, two new kinds of ionic vinylene-linked covalent organic frameworks (named as COF-NUC-1 and COF-NUC-2) are synthesized by novel geomimetic interfacial hydrothermal synthesis for the first time.

Sesquiterpene lactones from Cichorium intybus exhibit potent anti-inflammatory and hepatoprotective effects by repression of NF-κB and enhancement of NRF2.

Ethnopharmacological Relevance: Cichorium intybus is a traditional medicinal herb for hepatitis treatment in China and Europe. Sesquiterpene lactones are the main active ingredients in C. intybus.

Physiological Responses and Metabolic Characteristics of Proso Millet Under Drought Stress During Germination Period.

To clarify the impact of drought stress during germination on proso millet's physiological responses and metabolic features, this study used physiological and targeted-like metabolomics methods. With Longmi No. 7 (drought-tolerant, L1) and Longmi No.

A phenotype-based AI pipeline outperforms human experts in differentially diagnosing rare diseases using EHRs.

Rare diseases, affecting ~350 million people worldwide, pose significant challenges in clinical diagnosis due to the lack of experienced physicians and the complexity of differentiating between numerous rare diseases. To address these challenges, we introduce PhenoBrain, a fully automated artificial intelligence pipeline. PhenoBrain utilizes a BERT-based natural language processing model to extract phenotypes from clinical texts in EHRs and employs five new diagnostic models for differential diagnoses of rare diseases.

RsWOX13 promotes taproot development by activating cell division and expansion and sucrose metabolism in radish.

Radish is an important annual root vegetable crop, whose yield is largely dependent on taproot thickening and development. However, the regulatory network of WOXs-mediated taproot development remains poorly understood in radish. Herein, the RsWOX13 was classified in an ancient clade of the WOX gene family that harbors a conserved homeodomain.

Impact of oil-water emulsions on lubrication performance of ship stern bearings.

During maritime operations, extreme events such as explosions, grounding, and seal failures can cause water ingress into lubricant compartments, forming oil-water emulsions that significantly affect the lubrication performance of ship stern bearings. Existing studies mainly focus on low water content, with limited exploration of the impact of high water content on lubrication performance. To address this gap, viscosity measurements of oil-water mixtures were conducted, and an emulsification viscosity equation applicable to varying water contents was derived.

Action Potential Firing Patterns Regulate Dopamine Release via Voltage-Sensitive Dopamine D2 Autoreceptors in Mouse Striatum In Vivo.

Dopamine (DA) in the striatum is vital for motor and cognitive behaviors. Midbrain dopaminergic neurons generate both tonic and phasic action potential (AP) firing patterns in behavior mice. Besides AP numbers, whether and how different AP firing patterns per se modulate DA release remain largely unknown.

[Impacts of freeze-thaw process on soil microbial nutrient limitation in slope farmlands of the Chinese Mollisol region].

Understanding the impacts of freeze-thaw action on soil microbial nutrient limitation can provide important support for sustainable utilization of black soil resources. We analyzed the impacts of freeze-thaw action on soil microbial nutrient limitation on a slope farmland located in a typical thick Mollisol region of Keshan County, Heilongjiang Province. We examined the responses of soil microbial nutrient limitation to soil erosion rates through measuring soil nutrient, soil microbial biomass, and soil enzyme activities before and after freeze-thaw under natural conditions, and estimated the soil erosion rates by Cs tracing technology.

Exploring the Molecular Mechanisms underlying SADI-S Improves Glucose Metabolism in Type 2 Diabetic Rats through Liver Transcriptomics and Proteomics Analysis.

Metabolic surgery could improve or even reverse type 2 diabetes mellitus (T2DM). Single-anastomosis duodenal-ileal bypass with sleeve gastrectomy (SADI-S) is one of the most effective metabolic surgeries for T2DM. However, the molecular mechanisms behind the SADI-S-induced T2DM improvement are not fully understood.

A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin ( Blanco) and Tobacco ( L.).

The epigenetic variation in protoplast regeneration is a topic that has attracted interest recently. To elucidate the role of DNA methylation in the regeneration of protoplasts from the ponkan (), this study employs the methylation-sensitive amplification polymorphism (MSAP) molecular marker technique to analyze changes in DNA methylation levels and patterns during the isolation and culture of protoplasts from ponkan and tobacco. Additionally, differential DNA methylation fragments are cloned, sequenced, and subjected to bioinformatics analysis.

Three new taxa of the lichen genus (Megasporaceae, Ascomycota) from China.

Two new species; Lun Wang & Y. Y. Zhang, Lun Wang & Y.

Effects of Rehydration on Bacterial Diversity in the Rhizosphere of Broomcorn Millet ( L.) after Drought Stress at the Flowering Stage.

This study aimed to elucidate responses of the bacterial structure and diversity of the rhizosphere in flowering broomcorn millet after rehydration following drought stress. In this study, the broomcorn millet varieties 'Hequ red millet' (A1) and 'Yanshu No.10' (A2), known for their different drought tolerance levels, were selected as experimental materials.

Construction of an upconversion luminescence composite nanoprobe for ratiometric single particle imaging detection of hydrogen peroxide in food.

Hydrogen peroxide (HO) is associated with diseases and food safety. Thus, it is essential to achieve sensitive and efficient detection of HO. Herein, a ratiometric luminescence composite nanoprobe was designed for single particle imaging sensing of HO by combining NaYbF:Er@NaYbF:Tm@NaGdF:Yb upconversion nanoparticles (UCNPs) with cyanine dye IR-628.

Construction of UCNPs-aptamer-AuNPs luminescence energy transfer probe for ratio detection of Staphylococcus aureus.

A ratio luminescence probe was developed for detecting Staphylococcus aureus (S. aureus) based on luminescence energy transfer (LET) using double-wavelength emission (550 nm and 812 nm) upconversion nanoparticles (UCNPs) as donor, gold nanoparticles (AuNPs) as acceptor and the aptamer for S. aureus as the specific recognition and link unit.

A complete reference genome of broomcorn millet.

Broomcorn millet (Panicum miliaceum L.), known for its traits of drought resistance, adaptability to poor soil, short growth period, and high photosynthetic efficiency as a C plant, represents one of the earliest domesticated crops globally. This study reports the telomere-to-telomere (T2T) gap-free reference genome for broomcorn millet (AJ8) using PacBio high-fidelity (HiFi) long reads, Oxford Nanopore long-read technologies and high-throughput chromosome conformation capture (Hi-C) sequencing data.

Two homozygous adjacent novel missense mutations in DYSF gene caused dysferlinopathy due to splicing abnormalities.

Dysferlinopathy is an autosomal recessive disorder caused by mutations in the DYSF gene. This study reported two homozygous adjacent missense mutations in the DYSF gene, presenting clinically with bilateral lower limb weakness and calf swelling. Two homozygous adjacent missense mutations in the DYSF gene may be associated with the development of dysferlinopathy, but the exact mechanism needs further investigation.

Effects of single-anastomosis duodenal-ileal bypass with sleeve gastrectomy on gut microbiota and glucose metabolism in rats with type 2 diabetes.

Background: Bariatric and metabolic surgery often leads to significant changes in gut microbiota composition, indicating that changes in gut microbiota after bariatric and metabolic surgery might play a role in ameliorating type 2 diabetes (T2D). However, the effects of single-anastomosis duodenal-ileal bypass with sleeve gastrectomy (SADI-S) on gut microbiota in T2D remain unclear.

Objectives: To investigate the effects of SADI-S on gut microbiota and glucose metabolism in T2D rats.

LncRNA CRNDE promotes hepatoma cell proliferation by regulating the metabolic reprogramming of M2 macrophages via ERK pathway.

Background: LncRNA colorectal neoplasia differentially expressed (CRNDE) was found to be an important regulator in many cancers. This project focuses on the function of CRNDE on macrophage metabolic reprogramming and Hepatocellular carcinoma (HCC).

Method: qRT-PCR and Immunofluorescence were used to analyze Arg-1, IL-10, CD163, CCL-18, CD206, and CRNDE expression in HCC tissues and macrophages.

T7 peptide-mediated co-delivery platform overcoming multidrug-resistant breast cancer: In vitro and in vivo evaluation.

P-glycoprotein (P-gp) overexpressed mutidrug resistance (MDR) is currently a key factor limiting the effectiveness of breast cancer chemotherapy. Systemic administration based on P-gp-associated mechanism leads to severe toxic side effects. Here, we designed a T7 peptide-modified mixed liposome (T7-MLP@DTX/SchB) that, by active targeting co-delivering chemotherapeutic agents and P-gp inhibitors, harnessed synergistic effects to improve the treatment of MDR breast cancer.