Publications by authors named "Lumare A"

This study evaluated the effects of commercially available antiseptic mouthrinses on human gingival fibroblast and keratinocyte behaviour and metabolism. Three mouthrinses containing essential oil (EO), chlorhexidine (CHX) and amine fluoride/stannous fluoride (AFSF), were tested in an in vitro study. Human gingival fibroblasts and keratinocytes were washed with 10% or 30% concentration of the commercial mouthrinses and their effects on cell adhesion and proliferation were investigated as well as the specific gene expression of markers involved in oral mucosa metabolism.

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Background: In 2013 the International Journal of Surgical Pathology published a case report of intrasplenic malignant mesothelioma (MM) in a 48-year-old man: it was the first report in literature describing a case of primitive intra-splenic MM, described without  a history of asbestos exposure.

Objective: To verify the possible past exposure to asbestos, ignored by the patient himself, by studying in depth his environmental and occupational history.

Methods: Information about the occupational and non-occupational history of the subject was collected by Experts of the Operational Unit of Occupational Health and Safety Control (UOC PSAL) of the Local Health Unit Umbria 1 - Perugia, using the Italian National Mesothelioma Register (ReNaM) questionnaire and guide lines; an inspection was  carried out at the past canning industry where the patient worked in the period 1982-1990 and material was taken to be analysed by MOCF and SEM.

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Objective: The purpose of the research was to evaluate the respect of the regulation on the prohibition of alcohol consumption in the building sector through an alcohol breath test campaign, and to acquire information on the alcohol consumption by the contractors.

Methods: In the course of the investigation which covered the period 2011-2013, in a sample of construction, it was decided to access with verification of the presence of alcoholic beverages, administration workers to an anonymous questionnaire on alcohol consumption, execution of alcohol breath test.

Results: The reached population consisted in 1635 subjects, 1040 of which Italians and 595 foreigners; the consumers of at least 0,5 AU/die resulted being 354 among Italians and 250 among foreigners (p<0,05), the main occasion of consumption was observed being the meal for 39,8% of subjects; wine, followed by beer, were recorded as the most consumed beverages.

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Background: Starting from a project aimed at assessing the carcinogenic risk in some industrial sectors covered by Local Health Authority No.1 (LHA) in Perugia (USL Umbria1, Italian acronym), worksites for abatement of materials containing asbestos (MCA) were examined in order to estimate respirable fibres exposure levels in workers. Abatement work is, indeed, a fast developing sector, but is not free from asbestos risks in the long-term, particularly carcinogenic risks; this kind of work therefore requires maximum effort in respecting prevention standards prescribed in the relative legislation.

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Cadmium (Cd), an ubiquitous environmental metal, mainly used for industrial purposes, may be toxic at level of the reproductive system. Testis tubular-based Sertoli cells (SC), play a major role in constituting the blood-testis barrier and provide a unique microenvironment for the genesis and differentiation of germ cells. Hence SC strictly control sperm qualitative and quantitative parameters.

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Crystal micro-morphology and dimension of silica particles could be responsible for the high prevalence of silicosis as recently found among goldsmiths. In the present study we investigated two samples of silica particles with different surface sizes and shapes for their capacity to induce changes in ECM component production. In addition we investigated if their different effects could be related to cytotoxicity and apoptotic effects.

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The small dimension and particle shape of silica in gypsum used to prepare moulds for lost wax casting might be responsible for the high prevalence of silicosis in gold jewellery. To test this hypothesis, human pulmonary epithelial cell (BEAS-2B) cultures were exposed to two samples of silica with different crystal micro-morphologies: Silica Powder (Silica P) which is used in casting gold jewellery, and no powder Silica (Silica F). Extracellular matrix (ECM) production was evaluated using radio-labelled precursors and quantified by RT-PCR analysis.

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Background: Silicosis is mediated by macrophages, their soluble mediators, and extracellular matrix molecules. In this study, we investigated the effects of silica and/or hyaluronate (HA) on several alveolar macrophage responses.

Methods: We evaluated glycosaminoglycan (GAG) production by radiolabeled precursors, nitric oxide (NO) release by its oxidation product, phagocytic activity by Candida albicans internalization, and the secretion of two fibrogenic cytokines, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta, by specific assays.

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Background: Previous studies show that macrophages, lung fibroblasts, and their soluble mediators are responsible for the onset and development of pulmonary fibrosis. This study was conducted to determine whether airway epithelial cells are also directly involved in response to fibrogenic agents and consequently in the pathogenesis of lung fibrosis. To verify the hypothesis, we determined whether silica acts directly on human bronchial epithelial cells by stimulating cytokine and growth factor release and by modifying matrix production.

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Background: Silicosis, a pneumoconiosis marked by interstitial pulmonary fibrosis, is caused by inhalation of free crystalline silica particles. When silica particles are injected into the lower lung, they are translocated across the epithelium into the interstitial space, where macrophage-derived growth factors affect lung fibroblast proliferation and collagen deposition. We hypothesized that silica may act directly on pulmonary fibroblasts modifying extracellular matrix (ECM) synthesis and that the effects of silica may be mediated by transforming growth factor-beta (TGFbeta) overproduction.

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The cause of the Premature Infant Anemia (PIA) is mainly an insufficient erythropoiesis, a shorter mean-life of the red cells and in increased hemolysis. On these basis of prophylaxis has been attempted by treating newborns with folic acid, alpha-tocopherol and vitamins B1, B2, B6 and C. 208 premature babies have been treated (birth weight higher than 1750 nr.

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