Effects of 10% formalin fixation on fixed lung volume and lung tissue shrinkage. A comparison of eleven laboratory species.

This species comparative study examined tissue shrinkage from a known physiologic lung volume through to the processed histologic section. Eleven mammalian species with body weights that spanned 3 orders of magnitude were studied. Air pressure-volume curves were determined to obtain total lung capacity (TLC) at 30 cmH2O.

Morphometry of in situ and lavaged pulmonary alveolar macrophages from control and ozone-exposed rats.

Effects of ambient levels of ozone on cell size and compartments were determined morphometrically for both in situ and lavaged pulmonary alveolar macrophages from rats exposed to filtered air or to filtered air with 0.60 ppm ozone. The ozone exposure was 8 hr/day for 3 days.

Dark CO-fixation and diurnal malic acid fluctuations in the submerged-aquatic Isoetes storkii.

In the leaves (but not corms) of the submerged aquatic Isoetes storkii malic acid concentration fluctuated from 22 μeg g FW in the evening to 171 μeg g FW in the morning. Associated with this was a change in titratable acidity of 152 μeg g FW between morning and evening. C carbon was fixed in both the light and the dark, though the amount of carbon fixed in the light was more than that fixed in the dark.

A comparative study of cell renewal after exposure to ozone or oxygen. Response of terminal bronchiolar epithelium in the rat.

Cell renewal patterns of the terminal bronchiolar epithelium of rats were examined during 7-day exposures to either 0.8 ppm of ozone or 80 per cent O2. Terminal bronchiolar epithelial cells were classified and counted in ciliated, nonciliated secretory (Clara), and nonciliated, nonsecretory categories.

Morphometric and morphologic evaluation of pulmonary lesions in beagle dogs chronically exposed to high ambient levels of air pollutants.

Beagle dogs (104) comprising one control and seven treatment groups were exposed 16 hours daily for 68 months to filtered air, raw or photochemically reacted auto exhaust, oxides of sulfur or nitrogen, or their combinations. After a further 32 to 36 months in clean air, morphologic examination of lungs by light microscopy, scanning electron microscopy, and transmission electron microscopy revealed two important exposure-related lesions. They were enlargement of air spaces in proximal acinar regions, with and without increases in the number and size of interalveolar pores, and hyperplasia of nonciliated bronchiolar cells.