Publications by authors named "Lukas Probst"
Article Synopsis
- Monkeypox virus (MPXV) causes mpox, a pox-like disease, and since May 2022, there's been a notable outbreak with increased human-to-human transmission outside its usual regions.
- Whole genome sequencing (WGS) is essential for tracking MPXV evolution, but existing methods face challenges related to mutations and varied viral clades.
- A new isothermal WGS method using Phi29 DNA polymerase achieves high genome coverage (88% to 100%) from both DNA and clinical samples and is effective for monitoring MPXV outbreaks in standard laboratories.
Influenza D virus (IDV) can infect various livestock animals, such as cattle, swine, and small ruminants, and was shown to have zoonotic potential. Therefore, it is important to identify viral factors involved in the broad host tropism and identify potential antiviral compounds that can inhibit IDV infection. Recombinant reporter viruses provide powerful tools for studying viral infections and antiviral drug discovery.
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- The study investigates how influenza C (ICV) and D (IDV) viruses interact with different host species, focusing on their potential for cross-species transmission and differences in infection patterns.
- Both viruses predominantly infect ciliated cells in the respiratory epithelium, but ICV's replication is significantly affected by temperature, while IDV shows consistent replication across different conditions.
- The research identifies 34 immune-related genes that respond differently to each virus depending on the host species and temperature, shedding light on the factors that influence host tropism and transmission potential.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2.
View Article and Find Full Text PDFFerroptosis has recently been identified as a mode of programmed cell death. However, little is yet known about the signaling mechanism. Here, we report that lipoxygenases (LOX) contribute to the regulation of RSL3-induced ferroptosis in acute lymphoblastic leukemia (ALL) cells.
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