[Genetic variability of synthesis feature of carotenoids in Streptomyces globisporus 1912].

Seventeen spontaneous and induced mutants, that acquired a new characteristic--the synthesis of beta-carotene and lycopene, were obtained from strain Streptomyces globisporus 1912. It was found that spontaneous mutants inherited more stably the acquired carotenogenesis as compared to induced ones. Synthesis of carotenoids by all isolated Crt+ Lcp+ cultures is a constitutive feature.

[Quantum-chemical basics of pharmacokinetics (literary review and own investigations)].

The work is devoted to the use of quantum-pharmacological approaches in pharmacokinetic investigations. The main objective of the pharmacological researches is to find new, more active and less toxic drugs. To date, such a search is carried out empirically.

[Bacteriocidal activity of Streptomyces cultures].

Bacteriocidal activity of metabolites synthesized by 17 plasmid-containing cultures of Streptomyces has been studied. These cultures were isolated from soils of Ukraine with different anthropogenic contamination. The cultures, in their majority (85.

[Construction of a new bifunctional vector which contains EGFP-gene].

A number of bifunctional vector structures (pTpE, pTpE5, pTpH2, pTpH7, pTpS1) with molecular dimension of 11.3 kb has been created. Vector plasmids can be replicated, sustainably maintained and expressed both in Gram-negative (Escherichia coli XL1 Blue) and in Gram-positive (Streptomyces levoris 165) cultures.

[Plasmids of streptomycetes strains isolated from soils of Ukraine with different anthropogenic loading].

Screening of plasmid DNA was carried out among 94 streptomycetes cultures which were isolated from the samples of Ukrainian soils with different anthropogenic contamination. Seventeen streptomycetes strains containing plasmid DNA were found. It is established that some cultures contain more than one plasmid (Streptomyces sp.

[Cloning of amplicon of chromosomal SCO1206 sequences of Streptomyces coelicolor A 3(2)].

With the help of polymerase cycle reaction a fragment of chromosome Streptomyces coelicolor A 3(2) was amplified which contains gene SCO1206 coding subunit proteins of polyketide synthase III. Cloning of amplified DNA in the structure of shuttle vectors pAX5a and pWHM4 in Escherichia coli XL1 Blue was done. Plasmids of some selected ampicillin-resistent clones were transformed into protoplasts of plasmidless variant of Streptomyces globisporus 1912.

[Cloning of PCR-copies of Streptomyces coelicolor A3(2) chromosome fragment].

Copies of chromosome Streptomyces coelicolor A3(2) fragment (gene coding polyketide syntase of III type) were obtained to use polymerase cycle reaction. Amplified DNA was cloned in shuttle vector pWHM4 on sites for HindIII and EcoRI. The restriction analysis of plasmid DNAs of transformants has demonstrated that molecular size of cloned DNA fragment was 1134 bp.

[Ishemic-reperfusion syndrome prophylaxis in general hypothermia in experiment].

The influence of general supercooling on rats were studied. The action of corvitin and pentoxiphillin on the prooxidant-antioxidant homeostasis for conditions of general supercooling were analysed. On the based of investigations results analysis of lipids peroxide oxidation indexes and antioxidant protection system in animals it were established, that corvitin in difference of pentoxiphillin give more significant protectory effect in conditions of ishemic-reperfusion syndrome.

[Restriction analysis of streptomyces plasmid pSS 181].

Restriction analysis of plasmid was carried out with the use of 7 enzymes. Presence of restriction sites for endonucleases II EcoRI, Pstl, BamHI, SalGI, and BgII was established. A unique restriction site for endonuclease EcoRI on the plasmid pSS181 was found out, but endonucleases HindIII and XbaI had no restriction sites on the plasmid.

[Stability of synthesis of type-II restriction endonucleases by streptomyces strains after their long-term storage].

Three streptomycete strains (Streptomnyces sp. 14, Streptomnzyces sp. 34, Streptomyces sp.

[Plasmids of Streptomyces sp T8].

Strain Streptomyces sp. T8 was isolated from a soil sample from radical rhizosphere of tomatoes. A number of morphological, cultural, physiological and biochemical properties of the strain were investigated.

[Investigation of Streptomyces plasmid pSS27].

A bank of Streptomyces plasmid pSS27 fragments was created on the basis of the vector pBluescript II SK(+) and a bifunctional vector pWHM4. Strains of Escherichia coli DH1 and JM109 were used as recipients of hybrid DNA. Selected transformants contained hybrid molecules of DNA with inserts of Streptomyces plasmid from 0.

[Pharmacological correction of energy metabolism disturbance in the inflammatory-dystrophic process in the periodontium].

The influence of lipoic acid on the energy homeostasis in rats with chronic generalized parodontitis has been investigated. Lipoic acid possesses the energy-saving activity, which is related to an increase in oxidative phosphorylation efficiency.

[Endophytic bacilli--producers of type II restriction endonucleases].

Two of thirteen bacillar strains isolated from the inner tissues of cotton plants were found to produce type II restriction endonucleases. The investigation of the site specificity of these enzymes showed that they are AsuI and Eco31I isoschizomers.

[Bsu50441--isoschizomer of endonuclease restriction AsuI].

Endonuclease of restriction of type II has been found in Bacillus subtilis B-5044 during testing of endophytic strains of cotton plants bacilli. The restrictase Bsu5044I hydrolysed DNA of M13mp18 phage in 4 sites; pUC19 DNA in 5 sites; pBR322 in 15 sites. There were a lot of sites restriction in DNAs of phages T7 and lambda.

[Restriction analysis of streptomyces plasmid PSS14].

The plasmid pSS14 having a unique restriction site for endonuclease BamH1 and several identification sites for different restrictases: 5 sites of BglI, 2 sites of BglII, 8 sites of Bsp120I and 3 sites of SacII has been found in the strain Streptomyces sp. 14. Basing on the results of plasmid DNA of pSS14, its molecule weight 13.

[Restriction analysis of plasmid of the strain Streptomyces sp.27].

Plasmid pSS27 was found in streptomycete strain isolated from the soil sample in the Kyiv region. The paper deals with the data of restriction analysis of streptomycete plasmid pSS27 and its molecular weight. Plasmid pSS27 has unique restriction sites for endonucleases BglII and EcoRI, there are also the restriction sites for SalGI (3 sites), PstI (4 sites), BglI (5 sites), Bsp120I (7 sites) and SacII (11 sites).

[Type-II site-specific restriction endonucleases in endophytic strains of the genus Bacillus].

Using a modified Belavin's method the site-specific II type restriction endonucleases were determined for two endophytic strains of the Bacillus genus from the inner tissues of cotton plants.

[The determination of the site specificity of type-II restriction endonuclease in Streptomyces sp. 48].

The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages.

[The role of cAMP phosphodiesterase inhibitors in the pharmacotherapy of the hypoxic syndrome].

The authors studied the pharmacotherapeutic activity of drugs belonging to various pharmacological groups which are inhibitors of cAMP phosphodiesterase in the hypoxic syndrome. The increase in the level of the secondary intermediate of the adenylate cyclase path in transmission of the intracellular signal from the outer surface of the cell to the nucleus, the hypoxia protector cAMP, under the effect of the drugs under study favorably influenced survival and the course of hypoxic hypoxia in overheating. A combination of quercetin bioflavonoid with acetylsalicylic acid demonstrated stronger antihypoxic and thermoprotector properties.

[A search for site-specific type-II restriction endonucleases in streptomycetes].

Three Streptomycetes strains producing endonucleases of restriction of II type have been found using the modified method of Belavin.

[Antihypoxants: their status and outlook].

The survey deals with the up-to-date information in the literature on the problem of pharmacological correction of pathological changes found in the organism in the hypoxic syndrome. The principal approaches to pharmacotherapy of this pathological condition are pointed out. Information on the up-to-date and effective antihypoxia agents applied in medical practice and those in the stage of development is given.

[A biochemiluminescent analysis of the pharmacotherapeutic activity of acetylsalicylic acid in combination with quercetin in a hypoxic syndrome].

The effect of acetylsalicylic acid in combination with quercetin on blood serum biochemiluminescence in the hypoxic syndrome was studied. Possessing marked antioxidant activity, this drug combination prevented changes in the kinetics of blood serum luminescence in all periods of the investigation.

[The possible mechanisms of the antioxidant action of calcium channel blockers in the hypoxic syndrome].

The influence of calcium channel blockers on pro-oxidant-antioxidant balance in hypoxic syndrome is studied. Both verapamil and cinnarizine were shown to prevent formation and accumulation of lipid peroxidation products in the tissues and normalize the basic components of the system of antioxidant protection. Probable mechanisms of the antioxidant action of the calcium channel blockers are discussed.