Synthesis of hexyl butyrate (apple and citrus aroma) by lipase immobilized on Diaion HP-20 using the Box-Behnken design.

Unlabelled: This study aims at the synthesis of hexyl butyrate by lipase (CRL) immobilized on Diaion HP 20. The lipase load used was 28.7 ± 2.

Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters.

Objectives: To immobilize Candida rugosa lipase in Accurel MP 1000 (CRL-AMP) by physical adsorption in organic medium and apply in the synthesis of wax esters dodecanoyl octadecanoate 1 and hexadecanoyl octadecanoate 2 in a heptane medium, as well as evaluating the stability and recyclability of CRL-AMP in six reaction cycles.

Results: The specific activity (A) for CRL-AMP was 200 ± 20 U mg. Its catalytic activity was 1300 ± 100 U g.

High-throughput screening for distinguishing nitrilases from nitrile hydratases in Aspergillus and application of a Box-Behnken design for the optimization of nitrilase.

Nitrilases and nitrile hydratases/amidases hydrolyze nitriles into carboxylic acids and/or amides, which are used in industrial chemical processes. In the present study, 26 microorganisms, including yeasts and filamentous fungi, in a minimum solid mineral medium supplemented with glucose and phenylacetonitrile were screened to evaluate their biocatalytic potential. Of these microorganisms, five fungi of the genus Aspergillus were selected and subjected to colorimetry studies to evaluate the production and distinction of nitrilase and nitrile hydratase/amidase enzymes.

Optimization of lipase production by ATCC 10110 through solid-state fermentation using agro-industrial residue based on a univariate analysis.

Lipases (triacylglycerol hydrolases, EC 3.1.1.

Development of procedure for sample preparation of cashew nuts using mixture design and evaluation of nutrient profiles by Kohonen neural network.

A procedure using ICP OES for sample preparation for the determination of copper, iron and manganese in cashew nuts was developed. Constrained simplex-centroid design was applied in the optimization of the digestion in microwave oven procedure, and the results evaluated from topological maps of the Kohonen network. The best proportion evaluated for the digestion of the sample with HNO, HO and HO was 10:45:45 (%).