Comparative analysis of uninduced and neuronally-induced human dental pulp stromal cells in a 6-OHDA model of Parkinson's disease.

Background: In recent years, dental pulp stromal cells (DPSCs) have emerged as a promising therapeutic approach for Parkinson's disease (PD), owing to their inherent neurogenic potential and the lack of neuroprotective treatments for this condition. However, uncertainties persist regarding the efficacy of these cells in an undifferentiated state versus a neuronally-induced state. This study aims to delineate the distinct therapeutic potential of uninduced and neuronally-induced DPSCs in a rodent model of PD induced by 6-Hydroxydopamine (6-OHDA).

Mesenchymal stromal cells derived from exfoliated deciduous teeth express neuronal markers before differentiation induction.

Objective: This study aimed to evaluate neuronal markers in stromal cells from human exfoliated deciduous teeth (SHED) and standardize the isolation and characterization of those cells.

Methodology: Healthy primary teeth were collected from children. The cells were isolated by enzymatic digestion with collagenase.

Weight loss modifies lipid peroxidation and symmetric dimethylarginine levels in obese dogs.

In obese dogs, oxidative stress is associated with inflammatory processes and systemic endocrine imbalances. Monitoring oxidative status is an early and valuable means of obesity control as it is a marker of weight loss, which leads to a reduction in oxidative status or injury potential. The objective of this study was to investgate the changes in the concentrations of thiobarbituric acid reactive substances (TBARS) and symmetric dimethylarginine (SDMA) in obese female dogs subjected to an 8-week weight-loss program.

Treatment of Chronic Kidney Disease with Extracellular Vesicles from Mesenchymal Stem Cells and CD133 Expanded Cells: A Comparative Preclinical Analysis.

Chronic kidney disease (CKD) is characterized by structural abnormalities and the progressive loss of kidney function. Extracellular vesicles (EVs) from human umbilical cord tissue (hUCT)-derived mesenchymal stem cells (MSCs) and expanded human umbilical cord blood (hUCB)-derived CD133 cells (eCD133) maintain the characteristics of the parent cells, providing a new form of cell-free treatment. We evaluated the effects of EVs from hUCT-derived MSCs and hUCB-derived CD133 cells on rats with CDK induced by an adenine-enriched diet.

Polypropylene mesh inhibits post-laminectomy compressive scar formation in rabbits: Histopathological and computational morphometric evaluation.

We evaluated the effect of polypropylene mesh placement on post-laminectomy compressive scar formation in rabbits. Twenty-two white male New Zealand rabbits were distributed into two groups (n = 11). In the control group, the animals underwent lumbosacral laminectomy, whereas in the mesh group, the rabbits were submitted to lumbosacral laminectomy followed by the attachment of a polypropylene mesh to the vertebrae by the application of N-butyl cyanoacrylate.

Morphometric and Molecular Muscle Remodeling after Passive Stretching in Elderly Female Rats.

Objectives: To determine the effects of three sessions of a passive stretching exercise protocol on the muscles of elderly female rats.

Methods: The effects of the stretching exercises on the soleus muscle were analyzed using immunohistochemistry [tissue inhibitors of matrix metalloproteinases (TIMP), the tumor necrosis factor-alpha (TNF-α), and the gene expression levels using real-time PCR of the transforming growth factor-beta 1 (TGF-β1), collagen type 1 (COL1), and collagen type 3 (COL3)]. Fifteen 26-month-old female Wistar rats were randomly divided into two groups, namely, Stretching (SG, n=8) and Control (CG, n=7).

Lung Tissue Damage Associated with Allergic Asthma in BALB/c Mice Could Be Controlled with a Single Injection of Mesenchymal Stem Cells from Human Bone Marrow up to 14 d After Transplantation.

Mesenchymal stem cell (MSC) research has demonstrated the potential of these cells to modulate lung inflammatory processes and tissue repair; however, the underlying mechanisms and treatment durability remain unknown. Here, we investigated the therapeutic potential of human bone marrow-derived MSCs in the inflammatory process and pulmonary remodeling of asthmatic BALB/c mice up to 14 d after transplantation. Our study used ovalbumin to induce allergic asthma in male BALB/c mice.

Systemic Infusion of Expanded CD133 Cells and Expanded CD133 Cell-Derived EVs for the Treatment of Ischemic Cardiomyopathy in a Rat Model of AMI.

Myocardial infarction is a leading cause of death among all cardiovascular diseases. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs), expanded CD133 cells, have promise as a therapeutic option for the treatment of ischemic areas after infarction. Recently, secreted membrane vesicles, including exosomes and microvesicles, have been recognized as new therapeutic candidates with important roles in intercellular and tissue communication.

Expanded CD133 Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction.

Pharmacological approaches are partially effective in limiting infarct size. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs) CD133 have opened new perspectives for the treatment of ischemic areas after infarction. This preclinical study evaluated the effect of intramyocardial transplantation of purified or expanded human umbilical cord blood-derived CD133 cells on the recovery of rats following acute myocardial infarction (AMI).

The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis.

 To evaluate the effect of mesenchymal stem cells (MSCs) on fertility in experimental retrocervical endometriosis.  A total of 27 New Zealand rabbits were divided into three groups: endometriosis, in which endometrial implants were created; mesenchymal, in which MSCs were applied in addition to the creation of endometrial implants; and control, the group without endometriosis. Fisher's exact test was performed to compare the dichotomous qualitative variables among the groups.

Acute effects of stretching exercise on the soleus muscle of female aged rats.

Unlabelled: It has been shown that stretching exercises can improve the flexibility and independence of the elderly. However, although these exercises commonly constitute training programs, the morphological adaptations induced by stretching exercises in aged skeletal muscle are still unclear.

Objective: To assess the acute effects of passive mechanical static stretching on the morphology, sarcomerogenesis and modulation of important components of the extracellular matrix of the soleus muscle of aged female rats.

Comparison of two surgical techniques for creating an acute myocardial infarct in rats.

Objective: To perform a comparative assessment of two surgical techniques that are used creating an acute myocardial infarc by occluding the left anterior descending coronary artery in order to generate rats with a left ventricular ejection fraction of less than 40%.

Methods: The study was completely randomized and comprised 89 halothane-anaesthetised rats, which were divided into three groups. The control group (SHAM) comprised fourteen rats, whose left anterior descending coronary artery was not occluded.

Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy.

The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model.