Investigation of adenosine A1 receptor-mediated β-arrestin 2 recruitment using a split-luciferase assay.

Adenosine A1 receptor (AAR) plays a prominent role in neurological and cardiac diseases and inflammatory processes. Its endogenous ligand adenosine is known to be one of the key players in the sleep-wake cycle. Like other G protein-coupled receptors (GPCRs), stimulation of AAR leads to the recruitment of arrestins in addition to the activation of G proteins.