Publications by authors named "Luisa Fonte-Oliveira"

Cytospins are important for evaluating fluids with very low cellularity such as cerebrospinal fluid (CSF). The aim of this study was to compare the CSF cytospin preparations obtained from automated and manual cytocentrifugation methods. A prospective case series was performed to analyze canine CSF samples using both centrifugation methods.

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Background: Collecting cytology samples and making simple diagnoses are skills taught in veterinary universities, mostly in a passive way. Simulators enhance practical skills learning, increasing student engagement through immersive activities. These strategies have not been carefully assessed in veterinary cytology.

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Article Synopsis
  • Immunolabeling on Romanowsky-stained cytology (RSC) slides shows limited effectiveness for phenotyping lymphomas in dogs and cats compared to matched cell blocks (CB).
  • The study revealed that RSC had lower detection rates for CD3+ lymphocytes and faced challenges in identifying T-cell populations, while PAX5 showed good agreement between RSC and CB.
  • Interobserver agreement was better with CB, and it was found that pre-analytical variables significantly impacted the immunohistochemistry results on RSC, making CB samples more reliable for immunophenotyping.
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