Identification of Small RNAs in Using High-Resolution Transcriptomics and Expression Profiling During Clavulanic Acid Production.

Small non-coding RNAs play a pivotal role in regulating various metabolic processes in both prokaryotic and eukaryotic organisms. However, knowledge about small RNAs (sRNAs) in () is scarce. This study aimed to use cutting-edge bioinformatics tools and a compendium of RNA-seq data to predict the potential coding of sRNAs that might be present in the genome of ATCC 27064.

Environmental Factors Modulate the Role of Sigma Factor in Clavulanic Acid Production in ATCC27064.

Sigma factors and sigma factor-related mechanisms control antibiotic production in . In this contribution, the gene was overexpressed in the wild-type strain of ATCC2764, yielding /pIORF21, to further evaluate its regulatory effect on clavulanic acid (CA) biosynthesis under different culture medium conditions. The overexpression, regulated under the constitutive promoter , led to 2.