Spindly and Bub3 expression in oral cancer: Prognostic and therapeutic implications.

Objectives: Bub3 and Spindly are essential proteins required for the activation and inactivation of the spindle assembly checkpoint, respectively. Here, we explored the clinicopathological significance and the therapeutic potential of the opposing roles of the two proteins in oral squamous cell carcinoma (OSCC).

Materials And Methods: Bub3 and Spindly expression was evaluated by immunohistochemistry in 62 tissue microarrays from OSCC and by real-time PCR in OSCC cell lines and in normal human oral keratinocytes.

Survival probabilities and trends for lip, oral cavity and oropharynx cancers in Northern Portugal in the period 2000-2009.

Background: Oral cancer represents a serious public health problem worldwide. Our aim was to analyse the survival probabilities and trends of patients presenting with lip, oral cavity and oropharynx cancers, who were residents in the north of Portugal.

Methods: Using cancer-registry data, we conducted a population-based study of lip, oral cavity and oropharynx cancers diagnosed in the period 2000-2009, among residents in the north of Portugal.

Prognostic Significance of Cyclins A2, B1, D1, and E1 and Numerical Aberrations in Oral Squamous Cell Carcinomas.

We analysed the expression of cyclins A2, B1, D1, and E1 by immunohistochemistry and numerical aberrations in gene by fluorescence in situ hybridization technique in 67 primary oral squamous cell carcinomas (OSCC). Cyclin A2 expression was observed in 54 (83.1%) tumours, cyclin D1 in 58 (89.

An immunohistochemical score to predict the outcome for oral squamous cell carcinoma.

Background: Oral cancer is a major public health problem worldwide, with a poor survival. Our aim was to evaluate several protein markers in oral squamous cell carcinomas (OSCC) and analyse their prognostic value on patient's survival.

Methods: We analysed the expression of EGFR, p53, p27, p16, cyclin D1, cyclin A2, COX-2, Ki-67, Bcl-2, VEGFR-1 and VEGFR-2, by immunohistochemistry on 67 primary OSCC.

histological evaluation of the surgical margins made by different laser wavelengths in tongue tissues.

Background: Lasers have become standard tools for the surgical treatment of oral lesions. The purpose of this study is to determine the surgical margins and histologically evaluate the tissue thermal effects induced by different types of surgical instruments.

Material And Methods: Cuts were made in pork tongues' mucosa with different lasers (Er:YAG at 2W with and without air / water spray and at 4W with and without air / water spray; CO2 at 3.

Oral soft tissue biopsies in Oporto, Portugal: An eight year retrospective analysis.

Background: The diseases that affect the oral cavity are wide and diverse, comprising a broad spectrum of either benign or malignant lesions. However, few histological-based studies were performed for the evaluation of oral cavity lesions, and very few directed to oral soft tissue pathology. The aim of this study was to carry out pioneering research, within a Portuguese population, to determine the frequency and characteristics of oral malignancies, potential malignant disorders, and soft benign tissues pathologies submitted for biopsy in a north Portugal (Oporto) hospital population.

Er:YAG Laser Assisted Treatment of Central Odontogenic Fibroma of the Mandible.

Central odontogenic fibroma is a very rare benign odontogenic tumour characterized by a fibrous mature stroma with variable strands or islands of inactive-looking odontogenic epithelium. Our aim is to report a case of a central odontogenic fibroma and describe the clinical usefulness of Er:YAG laser for the surgical treatment of this tumour. A 74-year-old woman presented with an expansive lesion located in a mandible with multilocular and mixed radiographic appearance.

Oral cancer awareness and knowledge among residents in the Oporto city, Portugal.

Objective: To assess the awareness and knowledge on oral cancer in a general population of Oporto city, in Portugal.

Methods: Face-to-face interviews were performed with 1116 individuals, resident in the city of Oporto. Participants' socio-demographic information, lifestyle habits and awareness, knowledge and beliefs on oral cancer were ascertained.

Aesthetic Depigmentation of Gingival Smoker's Melanosis Using Carbon Dioxide Lasers.

Melanic pigmentation results from melanin produced by the melanocytes present in the basal layer of the oral epithelium. One of the most common causes of oral pigmentation is smoker melanosis, a condition associated with the melanocyte stimulation caused by cigarette smoke. This paper aims to illustrate the use of a carbon dioxide laser in the removal of the gingival melanic pigmentation for aesthetic reasons in a 27-year-old female patient with history of a smoking habit.

Outcomes of invitational and opportunistic oral cancer screening initiatives in Oporto, Portugal.

Objective: To assess the utility of oral cancer screening for the early detection of oral potentially malignant disorders (OPMDs) and oral cancer (OC) in a population of Oporto city.

Material And Methods: We conducted an OC screening program in the city of Oporto between 2010 and 2011 using two methodologies of population recruitment: Volunteers attending an OC screening day recruited via a mass media campaign; and OC screening during dental visits to a public hospital in the Oporto City. Participants' socio-demographic information and life-style habits were ascertained by interviewers, and an oral mucosal examination (screen) was conducted by previously trained and calibrated group of 20 screeners.

An overview of the spindle assembly checkpoint status in oral cancer.

Abnormal chromosome number, or aneuploidy, is a common feature of human solid tumors, including oral cancer. Deregulated spindle assembly checkpoint (SAC) is thought as one of the mechanisms that drive aneuploidy. In normal cells, SAC prevents anaphase onset until all chromosomes are correctly aligned at the metaphase plate thereby ensuring genomic stability.

EMMPRIN expression in oral squamous cell carcinomas: correlation with tumor proliferation and patient survival.

The aim of our study was to explore the clinicopathological and prognostic significance of extracellular matrix metalloproteinase inducer (EMMPRIN) expression in oral squamous cell carcinomas (OSCC), and its relation with the proliferative tumor status of OSCC. We examined EMMPRIN and Ki-67 proteins expression by immunohistochemistry in 74 cases with OSCC. Statistical analysis was conducted to examine their clinicopathological and prognostic significance in OSCC.

Phosphorylated mammalian target of rapamycin is associated with an adverse outcome in oral squamous cell carcinoma.

Objectives: To evaluate the expression of phosphorylated mammalian target of rapamycin (p-mTOR) and phosphatase and tensin homolog deleted on chromosome TEN (PTEN) in oral squamous cell carcinomas (OSCCs) and relate them with clinicopathologic characteristics and outcome.

Study Design: We analyzed p-mTOR and PTEN protein expression by immunohistochemistry in 72 OSCCs. Multivariate analysis was conducted to examine their role in survival.

Oral cancer awareness and knowledge in the city of valongo, portugal.

We conducted a questionnaire survey among 602 subjects in order to analyze the awareness and knowledge on oral cancer among residents of the city of Valongo in Portugal. The cancer that most subjects were aware of was breast cancer (99%). Oral cancer was the least mentioned cancer (68.

Combined cytoplasmic and membranous EGFR and p53 overexpression is a poor prognostic marker in early stage oral squamous cell carcinoma.

Objective: Our aim was to evaluate the expression of several molecules that regulate growth, the cell cycle and signalling pathways including EGFR, p53, p16 and p27 in oral squamous cell carcinomas (OSCC). We examined their utility as prognostic markers by relating to clinicopathological characteristics and the clinical outcome.

Patients And Methods: Using tissue microarray technology, we analysed 67 primary OSCC and examined immunohistochemical expression of EGFR, p53, p16 and p27.

EGFR and Ki-67 expression in oral squamous cell carcinoma using tissue microarray technology.

Objective: Our aim was to validate the use of tissue microarrays (TMA) in oral squamous cell carcinomas (OSCC) to analyse epidermal growth factor receptor (EGFR) and Ki-67 expression. We also analysed the relationship that the expression of these markers may have with clinical, pathological and survival variables.

Patients And Methods: The study sample comprised 39 unselected patients diagnosed and treated for OSCC.