Aligning digital CD8 scoring and targeted next-generation sequencing with programmed death ligand 1 expression: a pragmatic approach in early-stage squamous cell lung carcinoma.

Aims: To study programmed death ligand 1 (PD-L1) expression, tumour-infiltrating T lymphocytes (TILs) and the molecular context in patients with early-stage squamous cell lung carcinomas (SCCs).

Methods And Results: The study included samples from 40 patients (discovery cohort) and 29 patients (validation cohort) diagnosed with early-stage SCC. PD-L1 immunohistochemistry (IHC) was performed with three commercially available clones (E1L3N, SP263 and SP142).

The ALK translocation in advanced non-small-cell lung carcinomas: preapproval testing experience at a single cancer centre.

Aims: To study the ALK translocation in patients with advanced non-small-cell lung carcinomas (NSCLCs) seen at a European cancer centre, and its association with EGFR mutations, KRAS mutations and MET amplification.

Methods And Results: The study included samples from 86 patients diagnosed with advanced NSCLC. ALK fluorescence in-situ hybridization (FISH) was performed using the ALK break-apart probe set (Vysis).

Lung transplantation from out-of-hospital non-heart-beating lung donors. one-year experience and results.

There is currently no method for preservation and functional evaluation of clinical out-of-hospital non-heart-beating lung donors (NHBLD) that can be applied practically and systematically in clinical lung transplantation programs. A new method of preservation and functional evaluation of the lung has been developed in NHBLD that is based on the knowledge of various experimental studies. Initially, the viability of lungs harvested this way was proved from preliminary functional and histologic tests.