Thermogelling hydrogel charge and lower critical solution temperature influence cellular infiltration and tissue integration in an ex vivo cartilage explant model.

Thermogelling hydrogels based on poly(N-isopropyl acrylamide) (p[NiPAAm]) and crosslinked with a peptide-bearing macromer poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol) (PdBT) were fabricated to assess the role of hydrogel charge and lower critical solution temperature (LCST) over time in influencing cellular infiltration and tissue integration in an ex vivo cartilage explant model over 21 days. The p(NiPAAm)-based thermogelling polymer was synthesized to possess 0, 5, and 10 mol% dimethyl-γ-butyrolactone acrylate (DBA) to raise the LCST over time as the lactone rings hydrolyzed. Further, three peptides were designed to impart charge into the hydrogels via conjugation to the PdBT crosslinker.

Frequency-Dependent Light Stimulation Effects on Performance During Vigilance Tasks on a Laptop.

Students, office workers, or other computer and mobile device users can suffer from decrements in alertness or productivity, but many intervention methods on these can be too distracting or even affect daily routines. Using heart rate (HR) to determine a fast and slow target frequency at which to oscillate light brightness stimulation on a laptop, thirty-six participants joined a cognitive task where we hypothesized that fast frequency stimulation would increase alertness and decrease relaxation, while slow frequency stimulation would have the opposite effects. We found that slow frequency stimulation produces a statistically significant delay in response time, users react more slowly (3.