CRISPR-prime editing, a versatile genetic tool to create specific mutations with a single nucleotide resolution in .

Leptospirosis, caused by pathogenic bacteria from the genus , is a global zoonosis responsible for more than one million human cases and 60,000 deaths annually. The disease also affects many domestic animal species. Historically, genetic manipulation of has been difficult to perform, resulting in limited knowledge on pathogenic mechanisms of disease and the identification of virulence factors.

In Silico Structural and Functional Characterization of HtrA Proteins of spp.: Possible Implications in Pathogenesis.

Leptospirosis is a zoonosis caused by the pathogenic bacteria of the genus . The identification of conserved outer membrane proteins among pathogenic strains is a major research target in elucidating mechanisms of pathogenicity. Surface-exposed proteins are most probably the ones involved in the interaction of leptospires with the environment.

Heterologous Expression of the Pathogen-Specific LIC11711 Gene in the Saprophyte Increases Bacterial Binding to Laminin and Plasminogen.

Leptospirosis is a febrile disease and the etiological agents are pathogenic bacteria of the genus . The leptospiral virulence mechanisms are not fully understood and the application of genetic tools is still limited, despite advances in molecular biology techniques. The leptospiral recombinant protein LIC11711 has shown interaction with several host components, indicating a potential function in virulence.

Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically Inactive Cas9 (dCas9).

Easy, practical, and affordable gene silencing techniques are constantly progressing, and genetic tools such as TALEs, RNAi, and CRISPR/Cas9 have emerged as new techniques for understanding the basic biology and virulence mechanisms of pathogenic organisms, including bacteria. Here, we describe one-step targeted gene silencing in Leptospira biflexa by using plasmids expressing catalytically inactive Streptococcus pyogenes Cas9 (dCas9) and a single-guide RNA (sgRNA) capable of pairing to the coding strand of a desired gene.

Medical applications of clustered regularly interspaced short palindromic repeats (CRISPR/Cas) tool: A comprehensive overview.

Since the discovery of the double helix and the introduction of genetic engineering, the possibility to develop new strategies to manipulate the genome has fascinated scientists around the world. Currently scientists have the knowledge andabilitytoedit the genomes. Several methodologies of gene editing have been established, all of them working like "scissor", creating double strand breaks at specific spots.