Autologous transplantation of spermatogonial stem cells (SSCs) isolated from cryopreserved testicular biopsies obtained before oncological treatment could restore fertility in male childhood cancer survivors. There is a clear necessity for propagation of the limited SSCs from the testicular biopsy prior to transplantation due to limited numbers of spermatogonia in a cryopreserved testicular biopsy. Still, there is no consensus regarding their optimal culture method.
View Article and Find Full Text PDFWe first record Carcinops troglodytes (Paykull) as a predator of Alphitobius diaperinus (Panzer) larvae in poultry houses in North and Northwest regions of the state of Paraná. Carcinops spp. are commonly recorded as predators of dipterans, and this record in poultry houses indicates the possibility of exploiting such predator for future studies aiming the development of management strategies for A.
View Article and Find Full Text PDFTrichogramma pretiosum Riley and the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae are efficient biological control agents and are thought to be used jointly. In here, we investigated if these entomopathogens could have any side-effects on T. pretiosum.
View Article and Find Full Text PDFWe report the first occurrence of Zoophthora radicans infecting adults of Gyropsylla spegazziniana Lizer & Trelles in a commercial Paraguay tea plantation (Ilex paraguariensis), in Cascavel, PR, Brazil. The fungus prevalence was high (90% of mortality), considered a natural epizooty.
View Article and Find Full Text PDFDiatomaceous earth (DE) is a potential alternative to control the lesser mealworm of poultry farms Alphitobius diaperinus (Panzer). Our study aimed to understand the role of some of the environmental and insect behavioral factors play on DE effectiveness, such as the substrate (chicken food and poultry house litter), temperature and DE repellent activity on lesser mealworm adults. Mortality was higher at the highest temperature (32 masculineC), and it increased with DE concentration (53 and 84% respectively, for concentrations of 86 and 172 g/m(2)) (P < 0.
View Article and Find Full Text PDFMite infestations to the culture of mate-tea frequently causes losses by the premature fall of the leaves. So, it is necessary to monitor the population of these arthropods, and to adopt management strategies for their control. The objective of this research was to evaluate the trustworthiness of presence-absence sampling for Oligonychus yothersi (McGregor) on mate-tea orchard.
View Article and Find Full Text PDFThis paper presents results on isolates of the entomopathogenic fungus Beauveria bassiana for Alphitobius diaperinus (Coleoptera: Tenebrionidae) control. Of the 30 isolates tested, four (CG 71, CG 152, UNIOESTE 4, and UNIOESTE 40) resulted in mortality rates >or= 40% within 10 days. These mortality rates could be considered high because of the resistance of these species to B.
View Article and Find Full Text PDFThe objective of this research was to analyze the populational dynamic of the phytophagous mites, as well as that of their natural predators in the plants Ilex paraguariensis St. Hil. (Aquifoliaceae).
View Article and Find Full Text PDFThis study was carried out to evaluate the influence of temperature and poultry litter on germination vegetative growth virulence and conidial production of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.
View Article and Find Full Text PDFThis paper records the occurrence of Lysiphlebus testaceipes (Cresson) attacking aphids in wheat plantation in Medianeira, in the west region of Paraná State, Brazil. This microhymenoptera was introduced and released by Embrapa Trigo, from 1978 to 1992 in the wheat production region at the state of Rio Grande do Sul, what suggests that individuals of this species may show high dispersal ability.
View Article and Find Full Text PDFNineteen Bacillus sphaericus Neide strains obtained in Brazil were evaluated in addition to a standard strain (2362) supplied by Pasteur Institute. Most strains were more efficient than the standard, and seven of them caused mortality equal to or higher than 80%, at a low concentration (7 x 10(2) spores/ml). Strain ESALQ MS6 was selected for formulation, since it showed better yield in liquid culture medium (3 x 10(9) CFU/ml).
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