Publications by authors named "Luis Eduardo Segura-Garcia"
Article Synopsis
- Researchers studied the genomic diversity of non-typhoidal Salmonella in raw chicken across three central Mexican states, analyzing 192 strains collected from various retail sources using whole-genome sequencing.
- Among the twenty identified serovars, Infantis, Schwarzengrund, and Enteritidis were the most prevalent, with variations in serovar distribution and antimicrobial resistance (AMR) genes linked to different states, years, seasons, and retail establishments.
- The study found a total of 145 virulence genes and classified strains into 32 virulotypes, linking several strains to significant SNP clusters that matched global isolates, providing insights for Salmonella epidemiology and risk management strategies.
An efficient method for the direct extraction of yeast genomic DNA from agave must was developed. The optimized protocol, which was based on silica-adsorption of DNA on microcolumns, included an enzymatic cell wall degradation step followed by prolonged lysis with hot detergent. The resulting extracts were suitable templates for subsequent qPCR assays that quantified mixed yeast populations in artisan Mexican mezcal fermentations.
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