The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells.

Background: Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. The cspA encodes the major cold-shock protein from Escherichia coli (CspA).

Endophytes, a Potential Source of Bioactive Compounds to Curtail the Formation-Accumulation of Advanced Glycation End Products: A Review.

Endophytes, microorganisms that live in the internal tissues and organs of the plants, are known to produce numerous bioactive compounds, including, at times, some phytochemicals of their host plant. For such reason, endophytes have been quoted as a potential source for discovering bioactive compounds, particularly, of medical interest. Currently, many non-communicable diseases are threatening global human health, noticeably: diabetes, neurodegenerative diseases, cancer, and other ailment related to chronic inflammation and ageing.

Antidiabetic activity of Piper auritum leaves in streptozotocin-induced diabetic rat, beneficial effect on advanced glycation endproduct.

Objective: To investigate the effect of hypoglycaemic, hypolipidemic, oxidative stress, insulin resistance and advanced glycation endproducts (AGEs) formation of hexane extract from Piper auritum.

Methods: The streptozotocin (STZ)-induced diabetic Wistar rats were treated with the hexane extract from Piper auritum leaves for 28 days and a set of biochemical parameters were studied including glucose level, total cholesterol, triglycerides, lipid peroxidation, liver and muscle glycogen, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. The liver function was observed by determining glucose-6-phosphatase, glucokinase and hexokinase activities, and the effect of the hexane extract on insulin level and protein glycation.

ATP-citrate lyase activity and carotenoid production in batch cultures of Phaffia rhodozyma under nitrogen-limited and nonlimited conditions.

ATP-citrate lyase (ACL) is the key cytoplasmic enzyme which supplies acetyl-CoA for fatty acids in oleaginous yeast. Although it has been suggested that fatty acid and carotenoid biosynthesis may have a common source of acetyl-CoA in Phaffia rhodozyma, the source for carotenoids is currently unknown. The purpose of this work was to analyze the development of ACL activity during batch cultures of P.

Continuous Cr(VI) removal by Scenedesmus incrassatulus in an airlift photobioreactor.

Cr(VI) removal by Scenedesmus incrassatulus was characterized in a continuous culture system using a split-cylinder internal-loop airlift photobioreactor fed continuously with a synthetic effluent containing 1.0mg Cr(VI) l(-1) at dilution rate (D) of 0.3d (-1).