Effect of the application of benzyladenine pulse on organogenesis, acclimatisation and endogenous phytohormone content in kiwi explants cultured under autotrophic conditions.

In traditional in vitro culture, explants grow enclosed in a non-ventilated vessel at high relative humidity with phytohormones continuously present and sucrose as the main energy source. Under such conditions explant growth is far from normal. In this paper, explants of Actinidia deliciosa were cultured in MS medium supplemented with sucrose, benzyladenine and gibberellic acid under autotrophic conditions in glass boxes flushed with air enriched with 600 microl l(-1) CO(2) for the first 20 days and then transferred to MS medium until the end of the culture period.

Ethylene biosynthesis and endogenous polyamines in relation to development of in vitro cultured kiwifruit explants.

The relationship between polyamines and ethylene is controversial because the degree of interference of one pathway with the other may differ according to species, stage of development and experimental procedure. In this paper, we modify ethylene biosynthesis by the addition of aminoethoxyvinylglicine (AVG) or 1-aminocyclopropane-1-carboxylic acid (ACC) and study how it affects polyamine content and development of kiwifruit explants (Actinidia deliciosa CS Liang. & AR Fergusson).

Influence of CO2 and sucrose on photosynthesis and transpiration of Actinidia deliciosa explants cultured in vitro.

Explants of Actinidia deliciosa Chev. Liang and Ferguson var. Hayward were cultured in controlled CO2 atmospheres in the presence of different sucrose concentrations.