Global and cell type-specific immunological hallmarks of severe dengue progression identified via a systems immunology approach.

Severe dengue (SD) is a major cause of morbidity and mortality. To define dengue virus (DENV) target cells and immunological hallmarks of SD progression in children's blood, we integrated two single-cell approaches capturing cellular and viral elements: virus-inclusive single-cell RNA sequencing (viscRNA-Seq 2) and targeted proteomics with secretome analysis and functional assays. Beyond myeloid cells, in natural infection, B cells harbor replicating DENV capable of infecting permissive cells.

Magnitude and kinetics of the human immune cell response associated with severe dengue progression by single-cell proteomics.

Approximately 5 million dengue virus-infected patients progress to a potentially life-threatening severe dengue (SD) infection annually. To identify the immune features and temporal dynamics underlying SD progression, we performed deep immune profiling by mass cytometry of PBMCs collected longitudinally from SD progressors (SDp) and uncomplicated dengue (D) patients. While D is characterized by early activation of innate immune responses, in SDp there is rapid expansion and activation of IgG-secreting plasma cells and memory and regulatory T cells.

An 8-gene machine learning model improves clinical prediction of severe dengue progression.

Background: Each year 3-6 million people develop life-threatening severe dengue (SD). Clinical warning signs for SD manifest late in the disease course and are nonspecific, leading to missed cases and excess hospital burden. Better SD prognostics are urgently needed.

Clinical Features and Neurodevelopmental Outcomes for Infants with Perinatal Vertical Transmission of Zika Virus, Colombia.

Transplacental transmission of Zika virus has been reported during all trimesters of pregnancy and might lead to central nervous system anomalies, including microcephaly. We report 3 cases of perinatal Zika infection identified during the epidemic in Colombia and provide detailed descriptions of clinical features, diagnosis, and neurodevelopmental outcome at 18 months of age (corrected).

Heterogeneity of dengue transmission in an endemic area of Colombia.

Population based serological surveys are the gold-standard to quantify dengue (DENV) transmission. The purpose of this study was to estimate the age-specific seroprevalence and the force of infection of DENV in an endemic area of Colombia. Between July and October 2014, we conducted a household based cross-sectional survey among 1.

Development and Validation of a Rapid Lateral Flow E1/E2-Antigen Test and ELISA in Patients Infected with Emerging Asian Strain of Chikungunya Virus in the Americas.

Since its 2013 emergence in the Americas, Chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of the disease, though currently lacking in clinics, is integral to enable timely care and epidemiological response. We developed a dual detection system: a CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) and a lateral flow test using high-affinity anti-CHIKV antibodies.

Host biomarkers are associated with progression to dengue haemorrhagic fever: a nested case-control study.

Objectives: Dengue represents the most important arboviral infection worldwide. Onset of circulatory collapse can be unpredictable. Biomarkers that can identify individuals at risk of plasma leakage may facilitate better triage and clinical management.

Characterization of the health condition of people convalescing from a dengue episode.

Introduction: Although dengue and its acute manifestations have been broadly described in the literature, the symptoms during the convalescence phase have so far been little studied. 

Objective: To describe the clinical manifestations of a population during the convalescence phase from a dengue episode. 

Materials And Methods: We conducted an observational study in individuals that were in the hospital after being serologically diagnosed with dengue.

[Biochemical alterations as prediction markers for the severity of illness in dengue fever patients].

Introduction: Dengue is the most important mosquito-borne infection in the world. There is evidence supporting the use of biochemical alterations as prediction tools for severity of illness in dengue.

Objective: To evaluate biochemical alterations as potential prediction markers for severity in dengue.

Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.

Background: Dengue fever and leptospirosis have partially overlapping geographic distributions, similar clinical presentations and potentially life-threatening complications but require different treatments. Distinguishing between these cosmopolitan emerging pathogens represents a diagnostic dilemma of global importance. We hypothesized that perturbations in host biomarkers can differentiate between individuals with dengue fever and leptospirosis during the acute phase of illness.

[Roll of antibodies antiplatelets in viral infection: a systematic review of literature].

Introduction: Thrombocytopenia is a frequent phenomenon in viral infections. Peripheral platelet destruction mediated by anti-platelet antibodies has been one of the proposed causal mechanisms.

Objective: Results were collected and analyzed from published studies on associations of human viral infections on anti-platelet antibodies and total platelet counts.

[Reducing hospitalization with the use of a dengue management algorithm in Colombia].

Objective: Assess the impact of a dengue management algorithm on the hospitalization rate of patients with suspected disease in a primary care health facility in an endemic area of Colombia.

Methods: A quasi-experimental study was conducted at the local hospital in Piedecuesta, Santander, Colombia, based on comparison of two periods (18 weeks each), before and after use of the algorithm. This included recommendations for clinical diagnosis of dengue and the planning of follow-up visits and hemograms, as well as criteria for hospitalization and the discontinuation of follow-up.

[The validity and reliability of an instrument for measuring the satisfaction of users suffering from acute febrile syndrome].

Objective: Determining the validity and reliability of a user satisfaction instrument applied to patients attending the emergency department and clinic who were suffering from acute febrile syndrome in Bucaramanga's Public Health Network.

Methods: A diagnostic technology assessment study was conducted between 2008 and 2009 on patients suffering from acute febrile syndrome. A telephone questionnaire was designed using 3 instruments.

Predictors of spontaneous bleeding in patients with acute febrile syndrome from a dengue endemic area.

Background: Spontaneous bleeding is a common complication of dengue and is associated with an increased mortality.

Objective: To evaluate early clinical manifestations and simple laboratory tests as predictors of spontaneous hemorrhage in patients with forms of acute febrile syndrome (AFS) such as dengue from an endemic area.

Study Design: A prospective cohort study was performed including 729 non-bleeding AFS patients who were enrolled during the first 4 days of disease.

[Evaluation of PANBIO rapid immunochromatographic cassette for dengue diagnosis in a Colombian endemic area].

Introduction: Traditional tests for dengue diagnosis are not useful during the first days of disease onset, because they require time and complex techniques or because they have low sensitivity. Objective. The reliability and precision was determined for the rapid test in cassette (PANBIO) for dengue diagnosis in acute and convalescent serum samples.

Biochemical alterations as markers of dengue hemorrhagic fever.

We evaluated biochemical alterations as predictors of dengue hemorrhagic fever (DHF). Patients with confirmed infection with dengue virus were prospectively evaluated for the first seven days of disease to determine their final clinical outcome. Serum samples taken 48-96 hours after onset of fever were used for biochemical tests.

[Association between glutathione peroxidase levels and clinical manifestations of dengue].

Background: Glutathione peroxidase (GP) can be used as a marker of oxidative stress in infectious diseases.

Aim: To evaluate the association between the levels of glutathione peroxidase (GP) and the manifestations and complications of dengue.

Patients And Methods: Between April 2003 and December 2004, 161 patients with dengue were prospectively evaluated.

[Complications associated to severe thrombocytopenia in patients with dengue].

Background: Thrombocytopenia is a distinctive characteristic of dengue; however, few studies have related thrombocytopenia with its clinical severity.

Aim: To identify the complications of dengue, associated to severe thrombocytopenia, defined as a platelets count below 50,000/mm3).

Patients And Methods: Patients with clinical and serologic (positive IgM) diagnosis of dengue, consulting at hospitals located in Santander, Colombia, during the period 1993-1998, were studied.

[Early indicators of severity in dengue virus infection].

Objective: To identify clinical markers of severity in dengue, different from those of major hemorrhage.

Design: Cross-sectional study.

Population And Methods: Patients with a diagnosis of dengue infection admitted to hospitals in Santander (Colombia) during the years 1993 to 1998 were studied.

[Effectiveness of early dipyrone administration on severity of dengue virus infection in a prospective cohort].

Objective: To evaluate the impact of dipyrone administration in the first days of the disease on the severity of dengue infection.

Materials And Methods: Prospective cohort study in adults (> 12 years) with dengue virus infection, confirmed by specific IgM titers in paired samples or isolation of the virus. The patients were enrolled in the first 96 hours of the disease.