Publications by authors named "Lugarini F"
Autophagy is a conserved intracellular degradation pathway that generates de novo double-membrane autophagosomes to target a wide range of material for lysosomal degradation. In multicellular organisms, autophagy initiation requires the timely assembly of a contact site between the ER and the nascent autophagosome. Here, we report the in vitro reconstitution of a full-length seven-subunit human autophagy initiation supercomplex built on a core complex of ATG13-101 and ATG9.
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- AZAs (azaspiracids) are newly discovered marine biotoxins from a dinoflagellate in the Mediterranean, with certain analogues not yet studied for their biological effects.
- Organisms (mussels) were fed these toxins for 21 days, showing significant bioaccumulation and various harmful effects, including impaired immune function and tissue damage, mainly in the digestive system.
- After a recovery period, most of the toxic effects reverted to normal, suggesting these biomarkers can indicate seafood contamination levels related to AZA ingestion.
Fibro-adipogenic progenitors (FAPs) are typically activated in response to muscle injury, and establish functional interactions with inflammatory and muscle stem cells (MuSCs) to promote muscle repair. We found that denervation causes progressive accumulation of FAPs, without concomitant infiltration of macrophages and MuSC-mediated regeneration. Denervation-activated FAPs exhibited persistent STAT3 activation and secreted elevated levels of IL-6, which promoted muscle atrophy and fibrosis.
View Article and Find Full Text PDFTo investigate the possible involvement of leptin signaling in lipopolysaccharide (LPS) anorexia, we compared the anorectic effect of LPS in genetically obese (fa/fa) Zucker rats and in their lean (Fa/?) counterparts. The effects of interleukin-1beta (IL-1beta) and muramyl dipeptide (MDP) were also tested. LPS [100 microg/kg body weight (BW)], IL-1beta (2 microg/kg BW) and MDP (2.
View Article and Find Full Text PDFA role for cyclooxygenase-2 in lipopolysaccharide-induced anorexia in rats.
Am J Physiol Regul Integr Comp Physiol
October 2002
Because nonselective cycloooxygenase (COX) inhibition attenuated anorexia after lipopolysaccharide (LPS) administration, we tested the ability of resveratrol (2.5, 10, and 40 mg/kg) and NS-398 (2.5, 10, and 40 mg/kg), selective inhibitors of the two COX isoforms COX-1 and -2, respectively, to attenuate LPS (100 microg/kg ip)-induced anorexia.
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