Quantitative tracing of typical herbicides and their metabolites in sorghum agrosystems for fate tendency and cumulative risk.

Elucidating the combined exposure of agrochemicals is essential for safeguarding human health and agroecosystem safety. A rapid and high-sensitivity UHPLC-MS/MS method was developed for simultaneous quantification of nine compounds in sorghum by an assembly-line optimization process with a limit of quantitation of 0.001 mg/kg.

Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water.

High-performance antibodies are core reagents for highly sensitive immunoassays. Herein, based on a novel hapten, a hybridoma secreting the high-affinity anti-ethirimol monoclonal antibody (mAb-14G5F6) was isolated with an IC value of 1.35 μg/L and cross-reactivity below 0.

Unveiling the impact of harvest time on Dioscorea opposita Thunb. cv. Tiegun maturity by NMR-based metabolomics and LC-MS/MS analysis.

Background: Dioscorea opposita Thunb. cv. Tiegun maturity (DM) is an important factor influencing its quality.

Highly Sensitive and Rapid Screening Technique for the Detection of Organophosphate Pesticides and Copper Compounds Using Bifunctional Recombinant TrxA-PvCarE1.

Enabling the detection of organophosphate pesticide (OP) residues through enzyme inhibition-based technology is crucial for ensuring food safety and human health. However, the use of acetylcholinesterase, the primary target enzyme for OPs, isolated from animals in practical production poses challenges in terms of sensitivity and batch stability. To address this issue, we identified a highly sensitive and reproducible biorecognition element, TrxA-PvCarE1, derived from red kidney beans and successfully overexpressed it in .

Comprehensive widely targeted metabolomics to decipher the molecular mechanisms of thunb. cv. Tiegun quality formation during harvest.

Thumb. cv. Tiegun is commonly consumed as both food and traditional Chinese medicine, which has a history of more than two thousand years.

High-throughput screening and quantitative analysis of hidden ingredients in plant protection products by GC-MS/MS.

Hidden ingredients in plant protection products (PPPs) threaten public health, food trade, and the environment. In this study, we developed a high-throughput screening method of 639 hidden ingredients in PPPs using GC-MS/MS in multiple reaction monitoring mode. Results showed that the qualitative criteria of retention time (t) shift and uncertainty of qualifier to quantifier ratio in the commercially available Shimadzu Smart Pesticides Database were set at < 0.

A rapid method for detecting bronopol in fresh fish, shrimp, crab, and shellfish samples using liquid chromatography-tandem mass spectrometry.

Fish farming plays a vital role in providing food, nutrition, and employment globally. However, this industry faces security challenges, necessitating the use of fungicides and preservatives, such as bronopol, to increase product yields. Bronopol (2‑bromo-2-nitropropan-1,3-diol; CAS:52-51-7) is widely used in various fields, including food production, cosmetics, and, more recently, aquaculture.

Development of a specific and sensitive method for the detection of glyphosate pesticide and its metabolite in tea using dummy molecularly imprinted solid-phase extraction coupled with liquid chromatography-tandem quadrupole mass spectrometry.

Glyphosate, a widely used herbicide, and its primary metabolite aminomethyl phosphonic acid have been found to cause environmental and ecological issues and threaten human health. The conventional pretreatment method was insufficient for the extraction, concentration, and enrichment of trace substances, resulting in poor specificity. Thus, our objective was to develop a method for glyphosate pesticide detection using dummy molecularly imprinted solid-phase extraction (DMI-SPE) combined with liquid chromatography-tandem quadrupole mass spectrometry (DMI-SPE-LC/MS/MS).

Production of a Monoclonal Antibody for the Detection of Forchlorfenuron: Application in an Indirect Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip.

In this study, a monoclonal antibody (mAb) specific to forchlorfenuron (CPPU) with high sensitivity and specificity was produced and designated (9G9). To detect CPPU in cucumber samples, an indirect enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold nanobead immunochromatographic test strip (CGN-ICTS) were established using 9G9. The half-maximal inhibitory concentration (IC) and the LOD for the developed ic-ELISA were determined to be 0.

Different dissipation potential and dietary risk assessment of tristyrylphenol ethoxylates in cowpea ecosystem in China.

Tristyrylphenol ethoxylates (TSPEOn) are widely used as inert ingredients in pesticide formulations in the world. However, the information on the dissipation behavior of different homologs TSPEOn in agro-products is lacking. To investigate the dissipation behavior of TSPEOn, a cowpea field experiment treated with TSPEOn at different doses was carried out in Guangdong province, China.

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching.

Balancing the risks and benefits of organophosphate pesticides (OPs) on human and environmental health relies partly on their accurate measurement. A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs (triazophos, parathion, and chlorpyrifos) in apples, turnips, cabbages, and rice. Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.

Residue and Risk Assessment of Fluopyram in Carrot Tissues.

This study describes the variation in residue behavior of fluopyram in soil, carrot root, and carrot leaf samples after the application of fluopyram (41.7% suspension, Bayer) by foliar spray or root irrigation at the standard of 250.00 g active ingredient per hectare (a.

Design and Characterization of a Novel Hapten and Preparation of Monoclonal Antibody for Detecting Atrazine.

This study provides the first design and synthetic protocol for preparing highly sensitive and specific atrazine (ATR) monoclonal antibodies (mAbs). In this work, a previously unreported hapten, 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, was designed and synthesized, which maximally exposed the characteristic amino group ATR to an animal immune system to induce the expected antibody. The molecular weight of the ATR hapten was 259.

Recent progress in organic-inorganic hybrid materials as absorbents in sample pretreatment for pesticide detection.

Sample pretreatment is essential for trace analysis of pesticides in complex food and environment matrices. Recently, organic-inorganic hybrid materials have gained increasing attention in pesticide extraction and preconcentration. This review highlighted the common organic-inorganic hybrid materials used as absorbents in sample pretreatment for pesticide detection.

A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly.

A rapid detection method is introduced for residual trace levels of triazophos in water and agricultural products using an immunoassay based on catalytic hairpin self-assembly (CHA). The gold nanoparticle (AuNPs) surface was modified with triazophos antibody and sulfhydryl bio-barcode, and an immune competition reaction system was established between triazophos and its ovalbumin-hapten (OVA-hapten). The bio-barcode served as a catalyst to continuously induce the CHA reaction to achieve the dual signal amplification.

Piezoelectric sensing of glucose oxidase activity of Aspergillus niger spores pretreated by different methods.

The development of Aspergillus niger (A. niger) spores as glucose oxidase (GOD) biocatalysts to produce gluconic acid is highly anticipated in the food industry. Herein, a piezoelectric sensor (PIS) method has been developed for the detection of GOD activity and better application of rapid screening of GOD activity in A.

Novel FeO@metal-organic framework@polymer core-shell-shell nanospheres for fast extraction and specific preconcentration of nine organophosphorus pesticides from complex matrices.

Herein, a novel core-shell-shell magnetic nanosphere denoted as FeO@ZIF-8@polymer was fabricated by sequential in situ self-assembly and precipitation polymerization for effective magnetic solid-phase extraction of nine organophosphorus pesticides (OPPs) from river water, pear, and cabbage samples. The integrated FeO@ZIF-8@polymer featured convenient magnetic separation property and excellent multi-target binding ability. More importantly, the functional polymer coating greatly improved the extraction performance of FeO@ZIF-8 for OPPs, thus facilitating the simultaneous determination of trace OPP residues in real samples.

Determination of acetylgestagens in animal-derived matrix samples using enhanced matrix removal lipid clean-up in combination with ultra performance liquid chromatography-tandem mass spectrometry.

A robust and confirmative method was established for the determination of six acetylgestagen residues, namely, flurogestone acetate (FGA), megestrol (MA), melengestrol acetate (MGA), chlormadinone acetate (CMA), medroxyprogesterone (MPA), and hydroxyprogesterone acetate (HPA) in animal-derived matrix samples by utilizing enhanced matrix removal lipid (EMR-lipid) clean-up in combination with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The analytes were extracted with acetonitrile, purified with a EMR-lipid cartridge, and separated with a reversed-phase C18 column. The limit of quantification (S/N ≥ 10) for CMA, FGA, HPA, MA, and MGA in all matrices was 0.

Palladium-catalyzed stereoselective (3 + 2) cycloaddition of vinylethylene carbonates with cyclic -sulfonyl ketimines.

A diastereoselective (3 + 2) cycloaddition of N-sulfonyl ketimines with vinylethylene carbonates (VECs) in the presence of Pd2dba3·CHCl3 and PPh3 has been developed. The reaction of various substituted VECs and diverse cyclic N-sulfonyl ketimines proceeded smoothly under mild conditions, giving highly functionalized oxazolidine frameworks in good to excellent yields with moderate to good diastereoselectivities. With the use of spiroketal-based diphosphine SKP as a chiral ligand, an asymmetric version of the current (3 + 2) cycloaddition was achieved, and chiral products were obtained in >99% ee in most cases.

Preparation of molecularly imprinted polymer with class-specific recognition for determination of 29 sulfonylurea herbicides in agro-products.

Molecularly imprinting polymers with high selectivity toward 29 sulfonylurea herbicides were synthesized by precipitation polymerization, using metsulfuron-methyl and chlorsulfuron as the template molecule, 4-vinylpyridine as the function monomer, divinylbenzene as the crosslinking agent, and acetonitrile as porogen. The imprinted polymers were characterized and measured by scanning electron microscopy (SEM) and equilibrium adsorption experiments. The molecularly imprinted polymers displayed specific recognition for the tested 29 sulfonylurea herbicides, and the maximum apparent binding capacity was found to be 18.

Dietary exposure and risk assessment of cyanide via cassava consumption in Chinese population.

The intake of cassava would probably induce adverse health effects since there are toxic cyanide in cassava. However, the risk assessment of cassava consumption has not been reported in China. Therefore, this paper aimed to evaluate the dietary risks of cassava cyanide and proposed a maximum residue limit (MRL) for cyanogenic glycosides (CNGs) in cassava.

Fraud vulnerability quantitative assessment of Wuchang rice industrial chain in China based on AHP-EWM and ANN methods.

Vulnerability assessment has been used in the food fraud mitigation based on the subjective judgement of industry participants and simple calculation. To have a more objective result, an improved vulnerability quantitative assessment method was proposed. The overall fraud vulnerability was described by the vulnerability of fraud factors and the health and economic impact of fraud incidents.

An overview on molecular imprinted polymers combined with surface-enhanced Raman spectroscopy chemical sensors toward analytical applications.

Surface-enhanced Raman spectroscopy (SERS) is a powerful and high-speed detection technology. It provides information on molecular fingerprint recognition with ultrahigh sensitive detection. However, it shows poor anti-interference capacity against complex matrices.