Publications by authors named "Ludmila Sebejova"
Article Synopsis
- The study investigated urinary concentrations of 14 trace elements in 711 individuals from the Czech Republic to understand their exposure levels, including toxic metals and essential elements, and their health impacts.
- Young adults had higher levels of toxic elements, while children showed elevated levels of essential ones, with dietary factors like seafood and mushroom consumption influencing trace element concentrations.
- A significant percentage of participants exceeded the recommended health-based guidance values for arsenic and essential trace elements like zinc, indicating potential health risks associated with both deficiencies and excesses in trace element intake.*
Increased wildfire activity increases the demands on fire rescue services and firefighters' contact with harmful chemicals. This study aimed to determine firefighters' exposure to toxic metal(loid)s and its association with the lipid profile. CELSPAC-FIREexpo study participants (including 110 firefighters) provided urine and blood samples to quantify urinary levels of metal(loid)s (arsenic, cadmium (Cd), mercury, and lead (Pb)), and serum lipid biomarkers (cholesterol (CHOL), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), and triglycerides (TG)).
View Article and Find Full Text PDFIntroduction: Benzotriazoles and benzothiazoles (BTs) are high-production volume chemicals as well as widely distributed emerging pollutants with potential health risk. However, information about human exposure to BTs and associated health outcomes is limited.
Objective: We aimed to characterise exposure to BTs among Czech men, including possible occupational exposure among firefighters, its predictors, and its associations with liver function, serum lipids and oxidative stress.
Introduction: The proportion of older adults within society is sharply increasing and a better understanding of how we age starts to be critical. However, given the paucity of longitudinal studies with both neuroimaging and epigenetic data, it remains largely unknown whether the speed of the epigenetic clock changes over the life course and whether any such changes are proportional to changes in brain aging and cognitive skills. To fill these knowledge gaps, we conducted a longitudinal study of a prenatal birth cohort, studied epigenetic aging across adolescence and young adulthood, and evaluated its relationship with brain aging and cognitive outcomes.
View Article and Find Full Text PDFThe CELSPAC - FIREexpo biomonitoring study investigates the long-term effects of chemical exposure on firefighters' wellness and fitness. It aims to provide science-based measures to minimize the health risks of the firefighting occupation. Here, we present the study design, cohort profile, and first results with respect to internal per- and polyfluoroalkyl substances (PFAS) and polycyclic aromatic hydrocarbons (PAH) levels in study participants.
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- Replication stress (RS) contributes to genomic instability and can trigger either apoptosis or senescence in various cancer cell lines, depending on their genetic makeup, particularly the status of TP53 and expression of lamin A/C (LA/C).
- The study found that all cancer cell types showed uneven chromatin condensation due to RS, marked by unrepaired DNA damage and structural abnormalities like ultra-fine anaphase bridges.
- Responses to RS varied significantly among cell lines, with those containing wild-type p53 and LA/C transitioning to senescence, while others with mutated p53 or low LA/C levels exhibited apoptosis, indicating that these factors critically influence cell fate after RS exposure.
The treatment of relapsed/refractory chronic lymphocytic leukemia (CLL) remains a challenging clinical issue. An important treatment option is the use of high-dose corticosteroids. The purpose of this clinical trial was to determine the efficacy and toxicity of an ofatumumab-dexamethasone (O-Dex) combination in relapsed or refractory CLL.
View Article and Find Full Text PDFAbnormalities in ATM and TP53 genes represent important predictive factors in chronic lymphocytic leukemia (CLL); however, the efficacy of CD20 targeting immunotherapy is only poorly defined in the affected patients. Therefore, we tested the in vitro response to ofatumumab (OFA) and rituximab (RTX) in 75 CLL samples with clearly defined p53 or ATM inactivation. Using standard conditions allowing complement-dependent cytotoxicity, i.
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- * While ATM defects are known to influence the prognosis of CLL, their predictive value, especially in reaction to fludarabine, is less clear.
- * Findings reveal that CLL cells with inactive ATM can still accumulate the p53 protein and activate p53-related genes effectively after fludarabine treatment, indicating a potential for normal responses in ATM-deficient cells.
ATM abnormalities are frequent in chronic lymphocytic leukemia and represent an important prognostic factor. Sole 11q deletion does not result in ATM inactivation by contrast to biallelic defects involving mutations. Therefore, the analysis of ATM mutations and their functional impact is crucial.
View Article and Find Full Text PDFPurpose: There is a distinct connection between TP53 defects and poor prognosis in chronic lymphocytic leukemia (CLL). It remains unclear whether patients harboring TP53 mutations represent a homogenous prognostic group.
Patients And Methods: We evaluated the survival of patients with CLL and p53 defects identified at our institution by p53 yeast functional assay and complementary interphase fluorescence in situ hybridization analysis detecting del(17p) from 2003 to 2010.