New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).

The representatives of the archamoebian genus Pelomyxa are amoeboid anaerobic protists that inhabit fresh-water anoxic sediments, and most of them are usually multinucleate. The cytoplasm of these unicellular organisms is highly complicated and contains numerous vacuoles of different types, as well as a wide range of prokaryotic endocytobionts, agglomerations of glycogen, lipids, etc. Among the great variety of cytoplasmic structures in P.

Ciliates from the faeces of the free-ranging dromedary from Oman: Morphology and molecular phylogeny.

Ciliates Infundibulorium cameli from the faeces of the free-ranging dromedary from Oman were studied using a set of methods of the light and immunofluorescence microscopy and molecular phylogeny. With the use of molecular genetic methods, it was confirmed that the cysts found in the samples simultaneously with trophozoites actually belong to the species I. cameli.

Ciliates from the intestine of zoo-kept black rhinoceros, with immunofluorescence microscopic and molecular phylogenetic investigation of Rhinozeta rhinozeta (Litostomatea).

The study of endobiotic ciliates from the intestines of various zoo mammals is important for revealing the influence of various factors on endobiont communities. In this paper we describe the species diversity of endobiotic ciliates from the faeces of the eastern black rhinoceros Diceros bicornis michaeli (male and two females, mother and daughter) kept in the zoo. Seven species of the ciliates were found, among them Rhinozeta rhinozeta, R.

Pelomyxa doughnuta sp. nov. (Archamoebea, Pelobiontida) with an unusual nucleus-glycogen association.

We described Pelomyxa doughnuta sp. nov. and examined it with the use of light, electron, and immunofluorescence microscopy as well as cytochemical methods.

Intestinal ciliates (Ciliophora) from the wild plains zebra (Equus quagga) in South Africa, with notes on the microtubule cytoskeleton organisation.

Investigation of the ciliate communities from the digestive tract of different wild vertebrates is important in context of host-specificity of different ciliate species and the detection of any cases of non-specific infection. Here we present a description and analysis of the fauna of ciliates (Litostomatea, Trichostomatia) inhabiting the intestine of the wild plains zebra (Equus quagga Boddaert, 1785) in South Africa. Nineteen species belonging to 12 genera of five families were found.

The nature and features of organization of reserve polysaccharides in three Pelomyxa species (Archamoebea, Pelobiontida).

The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa-P. palustris, P. belevskii, and P.

Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae.

We have obtained a sequence of the 18S rRNA gene of the species Polychaos annulatum (Penard 1902) Smirnov et Goodkov 1998 using the isolation of a single nucleus from an amoeba cell. Attempts to amplify the 18S rRNA gene from the DNA of this species by conventional PCR were not successful, so we applied the whole genome amplification of the nuclear DNA followed by NGS sequencing. The 18S rRNA gene was found among the resulting contigs.

Identification of Pelomyxa palustris Endosymbionts.

Pelomyxa palustris is a giant anaerobic/microaerobic amoeba, characterized by a number of exceptional cytological and physiological features, among them the presumed absence of energy producing organelles and the presence of endosymbiotic bacteria. These endosymbionts have been previously distinguished as: a large rectangular-shaped Gram-variable rod with a central cleft; a slender Gram-negative rod; and a slender Gram-positive rod. Using DNA extracted from P.