Publications by authors named "Ludmila Blascakova"
Lipoproteins are very attractive natural-based transport systems suitable for applications in diagnostics and cancer therapy. Low- and high-density lipoproteins (LDL, HDL) were selected for hypericin (hyp) delivery in cancer cells. Hyp was used, as it is a well-known model for hydrophobic molecules, in order to estimate the LDL and HDL transport efficacy.
View Article and Find Full Text PDFBackground: Low-density lipoproteins (LDL) were used as a natural drug delivery system for the transport of hypericin (Hyp) in the bloodstream of the chicken's chorioallantoic membrane model (CAM). Hyp was chosen as a model for hydrophobic drug used in photo-diagnosis and photo-treatments (PDT). The extravasation of the Hyp:LDL complexes for different concentration ratios and the redistribution of Hyp between different serum components were investigated with an innovative statistical treatment.
View Article and Find Full Text PDFSecond-derivative absorption spectroscopy was employed to monitor the response of effective symmetry of cytochromes a and a to the redox and ligation states of bovine cytochrome c oxidase (CcO). The Soret band π → π* electronic transitions were used to display the changes in symmetry of these chromophores induced by the reduction of CcO inhibited by the exogenous ligands and during catalytic turnover. The second derivative of the difference absorption spectra revealed only a single Soret band for the oxidized cytochromes a and a and cyanide-ligated oxidized cytochrome a.
View Article and Find Full Text PDFBackground: The study was focused on evaluating the possible correlation between biochemical, anthropometric, and genetic indicators of osteoporosis in postmenopausal women. The frequency of genotypes and differences in measured parameters were evaluated within two ethnically different groups of women in Slovakia.
Methods: The study included 310 postmenopausal women divided into non-Roma and Roma groups.
Article Synopsis
- This study examines how ethanol, pH levels, and silver nanoparticles (Ag NPs) affect the aggregation of the anticancer compound parietin in water and on metal surfaces.
- Researchers utilized UV-visible absorption, fluorescence, and Raman spectroscopy to analyze the behavior of parietin in different environments, highlighting its deprotonation and molecular aggregation.
- The study also explored how varying ethanol concentrations influence parietin's optical properties and characterized its interactions with Ag NPs using surface-enhanced optical techniques to determine ionization states and pKa values.