CRISPR-Based Editing of the Gene.

gene is shown to have numerous diverse functions in plant development, including the regulation of embryo morphogenesis and maturation, hypocotyl elongation, flowering transition, etc. However, the functions of orthologs in different plant species have not been extensively studied. In this study, we obtained a line of , a model leguminous plant, carrying the loss-of-function mutation in the gene, orthologous to , using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas9) genome editing system.

The Genes from the Intermediate Clade: Influence on the Somatic Embryogenesis in .

Transcription factors from the WOX family are well-known regulators of cell proliferation and differentiation in plants. Herein, we focused on several genes from the intermediate clade and checked their impact on somatic embryogenesis using the model legume object . As a result, we show that overexpression not only stimulates somatic embryogenesis in the embryogenic line, as it was shown previously, but can also induce somatic embryogenesis in the non-embryogenic line.

and Effects on the Embryogenic Callus Transcriptome in .

WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of gene overexpression on the embryogenic callus development and transcriptomic state in .

, and Transcription Patterns and Their Possible Functions in the Embryogenic Calli of .

CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the gene, stimulating somatic embryogenesis in .

Selection Signatures in the First Exon of Paralogous Receptor Kinase Genes from the Region of the L. Genome.

During the initial step of the symbiosis between legumes (Fabaceae) and nitrogen-fixing bacteria (rhizobia), the bacterial signal molecule known as the Nod factor (nodulation factor) is recognized by plant LysM motif-containing receptor-like kinases (LysM-RLKs). The fifth chromosome of barrel medic ( Gaertn.) contains a cluster of paralogous LysM-RLK genes, one of which is known to participate in symbiosis.

Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root.

Background: Radish (Raphanus sativus L.) is a widespread agricultural plant forming storage root due to extensive secondary growth which involves cambium proliferation and differentiation of secondary conductive tissues. Closely related to the model object Arabidopsis thaliana, radish is a suitable model for studying processes of secondary growth and storage root development.

Horizontal gene transfer from Agrobacterium to plants.

Most genetic engineering of plants uses Agrobacterium mediated transformation to introduce novel gene content. In nature, insertion of T-DNA in the plant genome and its subsequent transfer via sexual reproduction has been shown in several species in the genera Nicotiana and Linaria. In these natural examples of horizontal gene transfer from Agrobacterium to plants, the T-DNA donor is assumed to be a mikimopine strain of A.

Horizontal gene transfer from genus agrobacterium to the plant linaria in nature.

Genes can be transferred horizontally between prokaryotes and eukaryotes in nature. The best-studied examples occur between Agrobacterium rhizogenes and certain Nicotiana spp. To investigate possible additional cases of horizontal gene transfer in nature between Agrobacterium and plants, a real-time polymerase chain reaction-based approach was employed to screen 127 plant species, belonging to 38 families of Dicotyledones, for the presence of oncogenes homologous to the transfer DNA fragments (T-DNA) from both A.

Wuschel-related homeobox5 gene expression and interaction of CLE peptides with components of the systemic control add two pieces to the puzzle of autoregulation of nodulation.

In legumes, the symbiotic nodules are formed as a result of dedifferentiation and reactivation of cortical root cells. A shoot-acting receptor complex, similar to the Arabidopsis (Arabidopsis thaliana) CLAVATA1 (CLV1)/CLV2 receptor, regulating development of the shoot apical meristem, is involved in autoregulation of nodulation (AON), a mechanism that systemically controls nodule number. The targets of CLV1/CLV2 in the shoot apical meristem, the WUSCHEL (WUS)-RELATED HOMEOBOX (WOX) family transcription factors, have been proposed to be important regulators of apical meristem maintenance and to be expressed in apical meristem "organizers.

Molecular markers of inbred radish (Raphanus sativus var. Radicola Pers.) lines.

An approach which could be used for quick searches for RAPD markers is described for groups of radish lines with certain morphological traits. The lines are characterized by various morpho-physiological abnormalities, including tumor formation (lines 12, 19, and 21) and non-terminal development of the flower meristem as a variant of tumor growth (line 6). We found four markers which differentiate tumor radish lines 12, 19, and 21 from the others, and two which differentiate line 6.