Publications by authors named "Ludimila Santos Amaral"

Article Synopsis
  • Research highlights the potential of entomopathogenic nematodes (EPNs) like Heterorhabditis bacteriophora for controlling invertebrate pests, including snails with One Health implications.* -
  • The study examined the effects of exposure time (24 vs. 48 hours) of H. bacteriophora on metabolism in Biomphalaria glabrata, revealing increased uric acid and urea levels along with reduced total protein.* -
  • Snails exposed longer (48 hours) experienced more significant metabolic disruptions, indicating that exposure duration impacts the level of physiological harm and the overall health of these organisms.*
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Article Synopsis
  • The study focused on the impact of the nematode Heterorhabditis bacteriophora on the snail species Pseudosuccinea columella, which is involved in fasciolosis transmission.
  • Over 24 and 48-hour exposure periods, the snails exhibited significant mortality rates of 48.25% and 65.52%, respectively, indicating that they are susceptible to this nematode.
  • The research suggests that using H. bacteriophora as a biological control agent could be effective in managing P. columella populations, especially in regions affected by fascioliasis, aligning with World Health Organization recommendations.
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The gastropod Pseudosuccinea columella participates in the dissemination of Fasciola hepatica in the environment, acting as the main intermediate host of this parasite in Brazil. The present study sought to elucidate the possible pathogenic effects of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora on P. columella, by evaluating the influence of infection on alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentrations of total protein, uric acid, and urea in the snail's hemolymph.

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Schistosoma mansoni is a heteroxenous parasite, meaning that during its life cycle needs the participation of obligatory intermediate and definitive hosts. The larval development occurs in aquatic molluscs belonging to the Biomphalaria genus, leading to the formation of cercariae, which emerge to infect the final vertebrate host. For this reason, studies for control of the diseases caused by digenetic trematodes often focus on combating the snail hosts.

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Article Synopsis
  • Heterorhabditis bacteriophora, an entomopathogenic nematode, shows potential for biological control by infecting the freshwater snail Biomphalaria glabrata, which is a host for the Schistosoma mansoni parasite in Brazil.
  • The study revealed significant physiological changes in B. glabrata, such as decreased glucose levels and reproductive impairment due to infection, indicating a process called parasitic castration.
  • Although the infection didn't kill the snails, it reduced their reproductive performance, suggesting H. bacteriophora could be used in controlling B. glabrata populations.
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Background: Angiostrongylus cantonensis is a metastrongylid nematode that has a heteroxenous cycle, where snails act as intermediate hosts and the rodents Rattus rattus and Rattus novergicus are the definitive hosts. However, humans may act as accidental hosts presenting an atypical form of parasitism. This fact has motivated research to better understand systems of relationships involving A.

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The effect of experimental infection by different parasitic loads of Angiostrongylus cantonensis (Nematode, Metastrongylidae) on the activities of the aminotransferases and concentration of total proteins, uric acid and urea in the hemolymph of Achatina fulica (Mollusca, Gastropoda) were investigated. There was a significant decrease in the concentration of total proteins in the exposed snails to 5000 or more larvae. This change was accompanied by an increase in the concentrations of urea and uric acid in the hemolymph, suggesting a higher rate of deamination of the amino acids.

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This study showed for the first time changes in the reproductive biology of Biomphalaria glabrata experimentally infected with Angiostrongylus cantonensis. The values of all the parameters analyzed (total number of eggs, number of egg masses, number of eggs/mass, number of eggs/snail, percentage of viable eggs and galactogen content in albumen gland) changed with progressive infection. The results indicate the occurrence of partial parasitic castration of B.

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