A highly selective cell-based fluorescent biosensor for genistein detection.

Genistein, an isoflavone found mainly in legumes, has been shown to have numerous health benefits for humans. Therefore, there is substantial interest in producing it using microbial cell factories. To aid in screening for high genistein producing microbial strains, a cell-based biosensor for genistein was developed by repurposing the Gal4DBD-ERα-VP16 (GEV) transcriptional activator in .

A -Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in .

In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in that is responsive to -coumaroyl-CoA, a central precursor of many flavonoids. The sensor is based on the transcriptional repressor CouR from and was applied in combination with a previously developed malonyl-CoA biosensor for dual regulation of -coumaroyl-CoA synthesis within the naringenin production pathway.

A single chromosome strain of S. cerevisiae exhibits diminished ethanol metabolism and tolerance.

Background: Eukaryotic organisms, like the model yeast S. cerevisiae, have linear chromosomes that facilitate organization and protection of nuclear DNA. A recent work described a stepwise break/repair method that enabled fusion of the 16 chromosomes of S.

An SMC-like protein binds and regulates Caenorhabditis elegans condensins.

Structural Maintenance of Chromosomes (SMC) family proteins participate in multisubunit complexes that govern chromosome structure and dynamics. SMC-containing condensin complexes create chromosome topologies essential for mitosis/meiosis, gene expression, recombination, and repair. Many eukaryotes have two condensin complexes (I and II); C.

Melanoma Perception in People of Color: A Targeted Educational Intervention.

Background: Although melanoma is more common in non-Hispanic Whites, ethnic minorities face a greater risk of melanoma-related mortality, which may be partially attributed to presentation at atypical sites and a lack of awareness.

Objective: Our objective was to assess the effectiveness of a melanoma educational intervention targeted towards people of color.

Design: Participants received one of two scripted melanoma educational interventions in the summer of 2015.

Persistence of atopic dermatitis (AD): A systematic review and meta-analysis.

Background: Previous studies found conflicting results about whether childhood atopic dermatitis (AD) persists into adulthood.

Objective: We sought to determine persistence rates and clinical factors associated with prolonged AD.

Methods: A systematic review was performed in MEDLINE, EMBASE, Scopus, GREAT, LILACS, Web of Science, Academic Search Complete, and Cochrane Library.

Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models.

Aim: The aim of this work is to evaluate combining targeting strategy and convection-enhanced delivery in brain tumor models by imaging quantum dot-immunoliposome hybrid nanoparticles.

Materials & Methods: An EGF receptor-targeted, quantum dot-immunoliposome hybrid nanoparticle (QD-IL) was synthesized. In vitro uptake was measured by flow cytometry and intracellular localization was imaged by confocal microscopy.

A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms.

Determining the localization, binding partners, and secondary modifications of individual proteins is crucial for understanding protein function. Several tags have been constructed for protein localization or purification under either native or denaturing conditions, but few tags permit all three simultaneously. Here, we describe a multifunctional tandem affinity purification (MAP) method that is both highly efficient and enables protein visualization.