Int J Biol Macromol
March 2024
Glutathione (GSH) production is of great industrial interest due to its essential properties. This study aimed to use machine learning (ML) methods to model GSHproduction under different growth conditions of Saccharomyces cerevisiae, namely cultivation time, culture volume, pressure, and magnetic field application. Different ML and regression models were evaluated for their statistics to select the most robust model.
View Article and Find Full Text PDFBioresour Technol
December 2023
This study aimed to produce carotenoids by Phaffia rhodozyma in a stirred-tank bioreactor under the influence of magnetic fields (MF) and to evaluate a sustainable approach to recover them from the yeast biomass. MF application proved to be effective in increasing 8.6 and 22.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
August 2023
This study aimed at estimating cultivation conditions to enable Yarrowia lipolytica NNRL Y-1095 to produce extracellular lipase and at evaluating the influence of magnetic fields (MF) on the lipase production and on its catalytic conditions. Culture conditions of carbon sources and surfactant defined to produce extracellular lipase were 10 g L glucose, 15 g L olive oil and 2 g L Triton X-100. The highest lipase activity (34.
View Article and Find Full Text PDFGlutathione (GSH) and peroxidase (POD) are biomolecules of interest in the global market; thus, it is desirable to seek ways to increase their production. Magnetic field (MF) application is one of the technologies used in cultivation that has shown promising results to increase bioproducts. Therefore, this study aimed at evaluating the influence of MFs on GSH and POD production by Saccharomyces cerevisiae ATCC 7754.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
September 2022
Several studies have shown that any magnetic field (MF) applied to microalgae modifies its cultivation conditions and may favor biomolecule production since it interacts with the microorganisms and affect their growth. As a result, there are changes in concentrations and compositions of biomass and biomolecules. This review aims at updating MF applications to microalga cultures that were reported by studies conducted in the last 5 years.
View Article and Find Full Text PDFGlutathione (L-γ-glutamyl-cysteinyl-glycine, GSH) is a tripeptide synthesized through consecutive enzymatic reactions. Among its several metabolic functions in cells, the main one is the potential to act as an endogenous antioxidant agent. GSH has been the focus of numerous studies not only due to its role in the redox status of biological systems but also due to its biotechnological characteristics.
View Article and Find Full Text PDFMicroalgae contribute significantly to the global carbon cycle through photosynthesis. Given their ability to efficiently convert solar energy and atmospheric carbon dioxide into chemical compounds, such as carbohydrates, and generate oxygen during the process, microalgae represent an excellent and feasible carbohydrate bioresource. Microalgae-based biofuels are technically viable and, delineate a green and innovative field of opportunity for bioenergy exploitation.
View Article and Find Full Text PDFThis study aimed to investigate the effects of magnetic field (MF) application (1, 12 and 24 h day) to Spirulina sp. LEB 18 in different photosynthesis cycles (dark and/or light) during short (15 days) and long periods (50 days) of cultivation. MF application was performed via two sources: ferrite magnets and solenoids.
View Article and Find Full Text PDFPeroxidase is an enzyme in the group of oxidoreductases that is widely distributed in nature. It can catalyze the oxidation of various organic and inorganic substrates by reacting with hydrogen peroxide and similar molecules. Due to its wide catalytic activity, peroxidases can act in the removal of both phenolic compounds and peroxides, in chemical synthesis and, according to recent studies, in mycotoxin degradation.
View Article and Find Full Text PDFThis study aimed to evaluate the influence of a magnetic field (MF) intensity of 25 mT on Chlorella fusca cultivation in outdoor and indoor conditions, and evaluate the changes in the macromolecules, pigment content and protein profile. C. fusca was cultivated for 15 d in raceway photobioreactor.
View Article and Find Full Text PDFChem Phys Lipids
May 2021
This study describes the physicochemical properties of soybean asolectin (ASO) liposomes loaded with phycocyanin (Phy) extracted from Spirulina sp. LEB 18. The effects of Phy in the liposomes' properties were investigated by Fourier transform infrared spectroscopy (FTIR), H and P nuclear magnetic resonance (NMR), zeta (ζ)-potential, dynamic light scattering (DLS) and ultraviolet-visible (UV-vis) techniques.
View Article and Find Full Text PDFCrop yield can be raised by establishment of adequate plant stand using seeds with high germination ratio and vigor. Various pre-sowing treatments are adopted to achieve this objective. One of these approaches is the exposure of seeds to a low-to-medium level magnetic field (MF), in pulsed and continuous modes, as they have shown positive results in a number of crop seeds.
View Article and Find Full Text PDFThis study aimed at evaluating mitigation of nivalenol (NIV) in alcoholic fermentation with magnetic field application (MF). Mitigation was related to both the glutathione (GSH) redox molecule and the enzyme peroxidase (PO), which were synthesized by Saccharomyces cerevisiae US-05. Conditions under evaluation were NIV (0.
View Article and Find Full Text PDFThis study aimed to investigate the influence of different intensities (15, 30 and 60 mT) and exposure times (1 h d 24 h d) of magnetic fields (MF) on the stimulation of lipid synthesis by the microalga Chlorella homosphaera. The growth and biochemical characterization of protein, carbohydrate and lipid content were determined. Biomass concentration increased by 20.
View Article and Find Full Text PDFMagnetic field (MF) can interact with the metabolism of microalgae and has an effect (positive or negative) on the synthesis of molecules. In addition to MF, the use of pentose as a carbon source for cultivating microalgae is an alternative to increase carbohydrate yield. This study aimed at evaluating the MF application on the mixotrophic culture of Chlorella minutissima in order to produce carbohydrates.
View Article and Find Full Text PDFThis study evaluated the influence of the magnetic field on the chemical composition of Spirulina sp. LEB 18 and its digestibility and protein solubility. The highest protein digestibility of biomass was obtained at 30 °C and with 2.
View Article and Find Full Text PDFStatic magnetic fields (SMF) influence the metabolism of microorganisms, however, there is no knowledge explaining how SMF act in cells. This study aimed at evaluating the SMF (30 mT) effect on photosynthetic performance, growth and biomass composition of the cyanobacterium Arthrospira platensis SAG 21.99.
View Article and Find Full Text PDFThis study aimed to use different conditions of magnetic field (MF) application during Chlorella fusca cultivation and evaluate CO biofixation by the microalga through growth kinetics in addition to the biomass composition. For this purpose, we tested different MF intensities applied for 1 h d and for 24 h. Cultures exposed to the MF for 1 h d (in both intensities) had greater biomass concentrations (1.
View Article and Find Full Text PDFThis study aimed at evaluating whether a magnetic field (MF) affects the growth of Spirulina sp. when applied to it at different exposure times in indoor and outdoor culture systems. The effects of MF on chlorophyll content, medium consumption and protein profile were also investigated.
View Article and Find Full Text PDFStatic or modulated magnetic fields (MF) may interact with the biological system and affect the metabolism of microorganisms, such as their photosynthetic capacity or synthesis of carbohydrates. Their effects on microorganisms, which can be classified into inhibiting, stimulating and null, may be interpreted as the result of stress that cells undergo, thus, leading to responses through the same mechanisms. Biological effects of exposure to magnetic forces depend on magnetic intensity, frequency and exposure time.
View Article and Find Full Text PDFThis study aimed at applying different intensities (30 and 60mT) and exposure times (24 and 1hd) of MF to cultures of Chlorella kessleri and evaluated their effects on cell growth, proximate composition of biomass, pigment production and antioxidant activity. The condition of 60mT for 1hd stimulated biomass concentration of 83.2% by comparison with control culture (CC).
View Article and Find Full Text PDFSimultaneous production of amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) was carried out by Aspergillus niger in substrate of defatted rice bran in a rotating drum bioreactor (RDB) and studied by a 3 × 2 factorial experimental design. Variables under study were A. niger strains (A.
View Article and Find Full Text PDFThis study aimed at evaluating the influence of magnetic field on the growth and biomass composition of Spirulina sp., cultivated in vertical tubular photobioreactors. Magnetic fields of 5, 30 and 60mT generated by electric current and ferrite magnets were applied at different lengths of time.
View Article and Find Full Text PDFA strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose concentration (30-70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754.
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