Publications by authors named "Lucie Morand"
The hyperthermophilic archaeon Thermococcus gammatolerans can resist huge doses of γ-irradiation, up to 5.0 kGy, without loss of viability. The potential to withstand such harsh conditions is probably due to complementary passive and active mechanisms, including repair of damaged chromosomes.
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- Tetrazolium salts, like tetrazolium violet, are commonly used in microbiology for staining bacteria since they produce a colored formazan product when reduced by bacteria.
- However, these salts can be toxic to bacteria, especially Salmonella, at high concentrations, affecting their growth and the staining process.
- The study found that adding magnesium to the growth medium with tetrazolium violet not only enhances the growth rate of Salmonella but also allows for growth at higher toxic concentrations of the salt, potentially improving detection methods for bacteria.
Foodborne pathogens are still a major concern for public health authorities. In this paper, we describe the optimization of a previously reported method which combines a highly specific capture of targeted food pathogens with an intracellular staining method. The reaction medium was optimized to simultaneously allow specific enrichment of Salmonella and maximize the staining of the target pathogen.
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