Publications by authors named "Lucie Kalferstova"
Article Synopsis
- * A comparative genome analysis of two distinct isolates of B. contaminans from a CF patient revealed significant genetic differences and changes in expression of virulence-related traits, indicating adaptations in a nutrient-rich environment.
- * The research is notable for being the first to explore the genomic and transcriptomic variations between longitudinal isolates of B. contaminans, suggesting a need for further understanding of its pathological potential in CF patients.
Article Synopsis
- Cepacia syndrome (CS) is a serious infection affecting about 20% of cystic fibrosis patients with Burkholderia cepacia complex infections, primarily caused by Burkholderia cenocepacia, especially strain ST32.
- A study analyzed gene expression in ST32 bacteria from the bloodstream during CS and compared it to earlier sputum samples, finding heightened virulence gene activity and a decrease in flagellar gene expression in blood isolates.
- Notably, a nonmotile form of B. cenocepacia was present in 6 out of 8 CS patients for up to 24 months before CS onset, suggesting that loss of motility could indicate an increased risk for developing CS.
Background: A highly transmissible Burkholderia cenocepacia sequence type (ST) 32 strain caused a major outbreak at the Prague Cystic Fibrosis (CF) Centre in the late 1990s and early 2000s. Because a large number of CF patients were affected by ST32, a rapid and easy-to-use diagnostic tool for ST32 infection was urgently needed for the detection of new cases as well as for long-term surveillance. The present study sought to identify unique DNA sequences within the ST32 genome to develop an ST32 strain-specific PCR assay.
View Article and Find Full Text PDFBackground: Once the outbreak with Burkholderia cenocepacia ST32 was identified in the Prague cystic fibrosis (CF) centre, molecular tools were implemented into diagnostic routine in order to complement infection control measures with as accurate as possible microbiological service. In addition, genotyping techniques were applied as part of an infection surveillance program to assign species and strain status in samples positive for Burkholderia cepacia complex (Bcc). We sought to evaluate a usefulness of Bcc-specific PCR in infection control and to map evolution of the outbreak.
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