From Alpha to Omicron BA.2: New digital RT-PCR approach and challenges for SARS-CoV-2 VOC monitoring and normalization of variant dynamics in wastewater.

Throughout the COVID-19 pandemic, new variants have continuously emerged and spread in populations. Among these, variants of concern (VOC) have been the main culprits of successive epidemic waves, due to their transmissibility, pathogenicity or ability to escape the immune response. Quantification of the SARS-CoV-2 genomes in raw wastewater is a reliable approach well-described and widely deployed worldwide to monitor the spread of SARS-CoV-2 in human populations connected to sewage systems.

[Nursing care and allogeneic hematopoietic stem cell transplantation.].

The management of an allogenic HCS raises a number of issues and challenges. The complexity of the field, the vital prognosis, exercise in a closed and protected environment, but also access to a field of knowledge whose scope and richness are constantly evolving require nurses to constantly update their knowledge and adaptation to the patient's needs and experiences.