Processing of matched and mismatched rNMPs in DNA by archaeal ribonucleotide excision repair.

Ribonucleoside monophosphates (rNMPs) are the main non-canonical nucleotides in genomic DNA, and their incorporation can occur as mismatches or matches . To counteract the mutagenic potential of rNMPs in DNA, all organisms evolved ribonucleotide excision repair (RER), a mechanism initiated by type 2 RNase H. Here, we describe the reconstitution of matched and mismatched rNMP repair using archaeal RER enzymes.