Publications by authors named "Luciano de S Vespoli"

Cadmium (Cd) is a heavy metal used as raw material for several fertilizers and pesticides. The increase of Cd concentration in soils has been observed in cultivated areas, affecting animals, plants, and microorganisms. Gluconacetobacter diazotrophicus is a plant growth-promoting bacterium able to survive under adverse environmental conditions.

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Article Synopsis
  • The study explores how the plant growth-promoting bacterium Gluconacetobacter diazotrophicus responds to salt stress, which is important for agriculture alternatives to mineral fertilizers.
  • Researchers found that at moderate salt concentrations (100 mM and 150 mM NaCl), the bacteria produced filamentous cells, but at higher concentrations, cell viability dropped significantly.
  • Proteomic analysis identified pathways affected by salt stress, particularly those involved in iron uptake, protein transport, and quality control, highlighting the essential role of the DegP protein in helping the bacteria tolerate salt stress.
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Microorganisms are constantly challenged by stressful conditions, such as sugar-rich environments. Such environments can cause an imbalance of biochemical activities and compromise cell multiplication. Gluconacetobacter diazotrophicus PAl 5 is among the most sugar-tolerant bacteria, capable of growing in the presence of up to 876 mM sucrose.

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Plant growth-promoting bacteria (PGPB) represent a promising alternative to the massive use of industrial fertilizers in agriculture. Gluconacetobacter diazotrophicus is a PGPB that colonizes several plant species. Although this bacterium is able to grow at high sucrose concentrations, its response to environmental stresses is poorly understood.

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Bacterial phylogenies have become one of the most important challenges for microbial ecology. This field started in the mid-1970s with the aim of using the sequence of the small subunit ribosomal RNA (16S) tool to infer bacterial phylogenies. Phylogenetic hypotheses based on other sequences usually give conflicting topologies that reveal different evolutionary histories, which in some cases may be the result of horizontal gene transfer events.

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