Dietary Influences on the Longevity and Reproductive Success of in Laboratory Studies: Sucrose vs. Honey.

Malaria continues to be a major public health challenge in tropical and subtropical regions. , a key laboratory model for malaria research, plays a critical role in the study of vector-parasite interactions. Although vector life traits and environmental factors such as age and resource availability can influence the transmission potential of mosquitoes for parasites, the impact of different adult diets on their survival and reproductive fitness remains underexplored.

Suitable Mouse Model to Study Dynamics of West Nile Virus Infection in Mosquitoes.

West Nile Virus (WNV) poses a significant global public health threat as a mosquito-borne pathogen. While laboratory mouse models have historically played a crucial role in understanding virus biology, recent research has focused on utilizing immunocompromised models to study arboviruses like dengue and Zika viruses, particularly their interactions with mosquitoes. However, there has been a shortage of suitable mouse models for investigating WNV and St.

The High Capacity of Brazilian Populations to Transmit a Locally Circulating Lineage of Chikungunya Virus.

The incidence of chikungunya has dramatically surged worldwide in recent decades, imposing an expanding burden on public health. In recent years, South America, particularly Brazil, has experienced outbreaks that have ravaged populations following the rapid dissemination of the chikungunya virus (CHIKV), which was first detected in 2014. The primary vector for CHIKV transmission is the urban mosquito species , which is highly prevalent throughout Brazil.

Numerical analysis of the mechanical behavior of ceramic dental implants based on Ce-TZP/AlO composite.

This study aimed to identify the potential use of the ceramic composite ZrO(CeO)-AlO as a dental implant due to its intrinsic geometry and different masticatory loads based on finite element simulations. Ceramic samples were sintered at 1500 °C-2h, and characterized: The mechanical properties of the ceramic composite (hardness, fracture toughness, flexural strength, Young's Modulus, and Poisson ratio) were determined, in addition to the relative density and its structural characteristics. Commercial dental implant designs (incisal and third-molar) on CAD models were used in this study as an initial implant geometry applied in a typical simulated mandible anatomy.

High capacity of Brazilian populations to transmit a locally circulating lineage of Chikungunya virus.

The global incidence of chikungunya has surged in recent decades, with South America, particularly Brazil, experiencing devastating outbreaks. The primary vector for transmitting CHIKV in urban areas is the mosquito species , which is very abundant in Brazil. However, little is known about the impact of locally circulating CHIKV genotypes and specific combinations of mosquito populations on vector competence.

Estimating the effect of the wMel release programme on the incidence of dengue and chikungunya in Rio de Janeiro, Brazil: a spatiotemporal modelling study.

Background: Introgression of genetic material from species of the insect bacteria Wolbachia into populations of Aedes aegypti mosquitoes has been shown in randomised and non-randomised trials to reduce the incidence of dengue; however, evidence for the real-world effectiveness of large-scale deployments of Wolbachia-infected mosquitoes for arboviral disease control in endemic settings is still scarce. A large Wolbachia (wMel strain) release programme was implemented in 2017 in Rio de Janeiro, Brazil. We aimed to assess the effect of this programme on the incidence of dengue and chikungunya in the city.

Response of Doru luteipes (Dermaptera: Forficulidae) to insecticides used in maize crop as a function of its life stage and exposure route.

The present study aimed to evaluate insecticide toxicity to Doru luteipes (Scudder), a major predator of maize pests. Lethal and sublethal effects were assessed on nymphs and adults exposed to the insecticides through contact (maize leaves) and ingestion (prey eggs) routes. Tested insecticides included a biopesticide (Spodoptera frugiperda multiple nucleopolyhedrovirus, SfMNPV), modern (flubendiamide and metaflumizone), and older neurotoxins (imidacloprid + β-cyfluthrin).

AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses.

Arboviruses (an acronym for "arthropod-borne virus"), such as dengue, yellow fever, Zika, and Chikungunya, are important human pathogens transmitted by mosquitoes. These viruses impose a growing burden on public health. Despite laboratory mice having been used for decades for understanding the basic biological phenomena of these viruses, it was only recently that researchers started to develop immunocompromised animals to study the pathogenesis of arboviruses and their transmission in a way that parallels natural cycles.

Experimental analysis and numerical simulations of the mechanical properties of a (Ce,Y)-TZP/AlO/H6A ceramic composite containing coupled toughening mechanisms.

Objectives: This study investigates the simulation of the mechanical behavior of a bioceramic composite based on (Ce,Y)-TZP reinforced with equiaxed AlO and platelet-shaped hexaaluminate (H6A) grains using Finit Element Method (FEM).

Methods: A commercial (Ce, Y)-TZP/AlO ceramic powder was compacted into disc-shaped specimens that were sintered at 1500 °C for 2 h. The sintered samples were further subjected to hydrothermal degradation in an autoclave at 134 °C, 0.

EVITA Dengue: a cluster-randomized controlled trial to EValuate the efficacy of Wolbachia-InfecTed Aedes aegypti mosquitoes in reducing the incidence of Arboviral infection in Brazil.

Background: Arboviruses transmitted by Aedes aegypti including dengue, Zika, and chikungunya are a major global health problem, with over 2.5 billion at risk for dengue alone. There are no licensed antivirals for these infections, and safe and effective vaccines are not yet widely available.

Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis.

In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet.

Multi-Omic Analysis of Symbiotic Bacteria Associated With Breeding Sites.

Mosquito breeding sites are complex aquatic environments with wide microbial diversity and physicochemical parameters that can change over time during the development of immature insect stages. Changes in biotic and abiotic conditions in water can alter life-history traits of adult mosquitos but this area remains understudied. Here, using microbial genomic and metabolomics analyses, we explored the metabolites associated with breeding sites as well as the potential contribution of sp.

Large-Scale Deployment and Establishment of Into the Population in Rio de Janeiro, Brazil.

Traditional methods of vector control have proven insufficient to reduce the alarming incidence of dengue, Zika, and chikungunya in endemic countries. The bacterium symbiont has emerged as an efficient pathogen-blocking and self-dispersing agent that reduces the vectorial potential of populations and potentially impairs arboviral disease transmission. In this work, we report the results of a large-scale intervention in Ilha do Governador, Rio de Janeiro, Brazil.

Wolbachia as translational science: controlling mosquito-borne pathogens.

In this review we examine how exploiting the Wolbachia-mosquito relationship has become an increasingly popular strategy for controlling arbovirus transmission. Field deployments of Wolbachia-infected mosquitoes have led to significant decreases in dengue virus incidence via high levels of mosquito population suppression and replacement, emphasizing the success of Wolbachia approaches. Here, we examine how improved knowledge of Wolbachia-host interactions has provided key insight into the mechanisms of the essential phenotypes of pathogen blocking and cytoplasmic incompatibility.

Effectiveness of Wolbachia-infected mosquito deployments in reducing the incidence of dengue and other Aedes-borne diseases in Niterói, Brazil: A quasi-experimental study.

Background: The introduction of the bacterium Wolbachia (wMel strain) into Aedes aegypti mosquitoes reduces their capacity to transmit dengue and other arboviruses. Evidence of a reduction in dengue case incidence following field releases of wMel-infected Ae. aegypti has been reported previously from a cluster randomised controlled trial in Indonesia, and quasi-experimental studies in Indonesia and northern Australia.

Reduced competence to arboviruses following the sustainable invasion of Wolbachia into native Aedes aegypti from Southeastern Brazil.

Field release of Wolbachia-infected Aedes aegypti has emerged as a promising solution to manage the transmission of dengue, Zika and chikungunya in endemic areas across the globe. Through an efficient self-dispersing mechanism, and the ability to induce virus-blocking properties, Wolbachia offers an unmatched potential to gradually modify wild Ae. aegypti populations turning them unsuitable disease vectors.

Mayaro Virus: The Potential Role of Microbiota and .

The Mayaro virus (MAYV) is an arbovirus that circulates mainly in tropical forests or rural areas in Latin America and is transmitted mainly by mosquitoes. The objective of this study was to evaluate the vector competence, microbiome, and the presence of in three populations infected with MAYV. The vector competence was assessed based on viral infection and transmission by RT-qPCR.

Evaluation of , , and Mosquitoes Competence to Infection.

The emergence of new human viral pathogens and re-emergence of several diseases are of particular concern in the last decades. (OROV) is an arbovirus endemic to South and Central America tropical regions, responsible to several epidemic events in the last decades. There is little information regarding the ability of OROV to be transmitted by urban/peri-urban mosquitoes, which has limited the predictability of the emergence of permanent urban transmission cycles.

Survival, development and reproduction of Harmonia axyridis (Pallas, 1773) (Coleoptera: Coccinellidae) under effects of insecticides used in tomato plants.

Agriculture depends on the correct use of different methods for pest control, however the cultivation methods adopted for Solanaceae demands numerous pesticide applications. These products can be either harmful or selective to beneficial organisms, such as Coccinellidae predators. The aim of this study was to assess the physiological selectivity of insecticides, registered for tomato pest control, on Harmonia axyridis (Pallas, 1773) (Coleoptera: Coccinellidae).

How to engage communities on a large scale? Lessons from World Mosquito Program in Rio de Janeiro, Brazil.

One of the pillars of the World Health Organization's (WHO) Global Vector Control Response 2017-2030 strategy is the engagement of communities. Among the priority activities, defined by 2022 by the WHO, is the development of plans for the effective engagement and mobilisation of communities in vector control. Novel technologies for arboviruses control are being developed, such as the method, implemented by the World Mosquito Program (WMP).

The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing.

Background: Aedes aegypti control programs have failed to restrain mosquito population expansion and, consequently, the spread of diseases such as dengue, Zika, and Chikungunya. Wolbachia infection of mosquitoes is a new and promising complementary tool for the control of arbovirus transmission. The use of Wolbachia-infected mosquitoes, mass reared using human blood, is currently being tested in several countries.

Dengue infection modulates locomotion and host seeking in Aedes aegypti.

Pathogens may manipulate their human and mosquito hosts to enhance disease transmission. Dengue, caused by four viral serotypes, is the fastest-growing transmissible disease globally resulting in 50-100 million infections annually. Transmission of the disease relies on the interaction between humans and the vector Aedes aegypti and is largely dependent on the odor-mediated host seeking of female mosquitoes.