Publications by authors named "Luciane A C Tonon"
Marine bacteria living in association with marine sponges have proven to be a reliable source of biologically active secondary metabolites. However, no studies have yet reported natural products from Microbacterium testaceum spp. We herein report the isolation of a M.
View Article and Find Full Text PDFArticle Synopsis
- The manuscript describes a new bacterial strain called A621, characterized as Gram-negative motile rods forming green circular colonies on TCBS, isolated from the skin of the sharpnose pufferfish in Brazil.
- Optimal growth for A621 occurs at temperatures of 20-28 °C with 3% NaCl, and its genome consists of 4.224 Mb with 4431 coding genes and a G+C content of 44.5%.
- Genomic analysis classifies A621 as a new species within the Vibrio genus, named Vibrio fluminensis sp. nov., and reveals gene clusters that indicate potential ecological roles in its fish host.
Bacterial genome sequencing has revealed a vast number of novel biosynthetic gene clusters (BGC) with potential to produce bioactive natural products. However, the biosynthesis of secondary metabolites by bacteria is often silenced under laboratory conditions, limiting the controlled expression of natural products. Here we describe an integrated methodology for the construction and screening of an elicited and pre-fractionated library of marine bacteria.
View Article and Find Full Text PDFArticle Synopsis
- A novel Gram-negative, motile bacterial strain (designated A511) was discovered from the skin of pufferfish collected in Brazil, specifically at 20-28 °C with 3% NaCl for optimal growth.
- The genome of this strain is 4.36 Mb containing 3,976 coding genes and a G+C content of 42.5%.
- Taxonomic analyses identified A511 as a new species in the Vibrio genus, named Vibrio tetraodonis sp. nov., and revealed gene clusters related to bioactive compounds that may have ecological roles in its pufferfish host.
Article Synopsis
- The study investigates whether bacteria from pufferfish can produce tetrodotoxin (TTX), a toxic substance commonly found in pufferfish tissues, raising safety concerns about consumption.
- UPLC qTOF-MS/MS analysis confirmed TTX presence in the tissues of Sphoeroides spengleri and Canthigaster figuereidoi, and identified new TTX analogs like methyl-TTX and TTX-acetate.
- Although a compound resembling TTX was found in bacterial extracts, the actual TTX was not detected, suggesting that certain Vibrio bacteria may be involved in producing TTX precursors rather than the toxin itself.
Article Synopsis
- A649 is a novel bacterial species isolated from the healthy pufferfish Sphoeroides spengleri, classified as Enterovibrio baiacu sp nov.
- Genomic analysis shows A649 has significantly low amino acid/nucleotide identity (less than 95%) and genome similarity (less than 70%) compared to its closest relatives.
- The complete genome of strain A649 is approximately 5.4 million base pairs long and contains 4,826 coding genes, with unique features for distinguishing it from other Enterovibrio species.
Article Synopsis
- - The genome sequence of Shewanella corallii strain A687 was obtained from a pufferfish species called Sphoeroides spengleri.
- - This genetic assembly is made up of 5,215,037 base pairs and includes 284 separate fragments known as contigs.
- - The G+C content of the genome is 50.3%, which indicates the percentage of guanine and cytosine nucleotides in the DNA.
Covering: up to 2019The discovery of new bioactive natural products gained momentum during the last few decades, resulting from instrumentation advances, from the expansion of genome mining and regulation, as well as by exploration of untapped biological sources. However, water-soluble, volatile, minor and photosensitive natural products are yet poorly known. This review discusses the literature reporting the isolation strategies for some of these metabolites.
View Article and Find Full Text PDFFour strains of lactic acid bacteria isolated from cachaça and alcohol fermentation vats in Brazil were characterised in order to determine their taxonomic position. Phylogenetic analysis revealed that they belong to the genus Oenococcus and should be distinguished from their closest neighbours. The 16S rRNA gene sequence similarity against the type strains of the other two species of the genus was below 94.
View Article and Find Full Text PDFThree novel isolates (A-354(T), A-328, and A-384) were retrieved from apparently healthy scleractinian Madracis decactis in the remote St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil. The novel isolates formed a distinct lineage based on the phylogenetic reconstruction using the 16S rRNA and pyrH gene sequences. They fell into the Mediterranei clade and their closest phylogenetic neighbour was V.
View Article and Find Full Text PDFCulturable heterotrophic bacteria associated with healthy and bleached scleractinian Madracis decactis and the fireworm Hermodice carunculata from the remote St. Peter and St. Paul Archipelago, Brazil.
Curr Microbiol
January 2014
We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remote St.
View Article and Find Full Text PDF