Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study.

This study aimed to assess the biological properties of two ready-to-use bioceramic sealers (EndoSequence BC Sealer - EBCS; Bio-C Sealer - BCS) on osteoblastic lineage cells. MC3T3 osteoblast-like cells were exposed to extracts of bioceramic materials. Cytotoxicity was evaluated using the MTT method, genotoxicity was assessed by the micronucleus test and the expression of BMP1, BMP2 and ALP was measured by RT-qPCR, after 1, 3 and 7 days.

Cytotoxicity and genotoxicity of Bio-C Repair, Endosequence BC Root Repair, MTA Angelus and MTA Repair HP.

The aim was to evaluate in vitro cytotoxicity and genotoxicity of Bio-C Repair (BCR), compared to Endosequence BC Root Repair (ERRM), MTA Angelus (MTA-Ang), and MTA Repair HP (MTA-HP). MC3T3 osteoblastic cells were exposed to extracts of the repairing bioceramic cements. After 1, 3, and 7 days, cytotoxicity and genotoxicity were evaluated by MTT and Micronucleus tests, respectively.

Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects-An In Vitro Study.

Objective: The aim of the study was to characterize and evaluate the stability, antimicrobial activity, cytotoxicity, and remineralizing effects of silver nanoparticles and fluoride anticaries agent (AgF) on staining dental enamel.

Materials And Methods: An experimental AgF solution was prepared and compared to silver diamine fluoride (SDF). First, the AgF was characterized and the stability was evaluated by transmission electron microscopy (TEM).

Evaluation of the effects of different applications of kinesio taping on postural control in healthy women.

Unlabelled: It has been demonstrated that Kinesio Tape (KT) application has immediate positive effects on balance in healthy individuals, but its mid-term effects have not yet been investigated.

Objective: Evaluate the effects of KT on postural control in young women, using four strategies.

Methods: Forty-eight healthy women aged 18-35 years old were randomly assigned to four groups with different elastic bandages (G1: ankle; G2: hamstrings; G3: lumbar; G4: different taping applications) and their postural control was evaluated using stabilographic parameters obtained on a force platform during right and left unipodal (UNP-R and UNP-L) and semi-tamdem tests at pre-intervention (PRE), immediately after (IME), and 24 and 48 h after the application of bandages.

Microcurrent stimulates cell proliferation and modulates cytokine release in fibroblast cells.

Aims: To analyse the effects of microcurrent on L929 fibroblast cell culture.

Methods: Cells were cultivated in six-well plates at densities of 5×10, 1×10, 3×10 and 5×10 cells/well to determine the best plating density. Subsequently, two methods of current application were tested: with a paper cone coupled to the electrode (M1) and with the electrode directly inside the well (M2).

Evaluation of Dental Practices Changes During the COVID-19 Pandemic in Brazil.

Dentists are healthcare professionals who are at high risk of infection and transmission of the coronavirus disease-19 (COVID-19). The primary objective of the present study was to evaluate the changes in clinical practice made by Brazilian dentists during the COVID-19 pandemic. From June 22 to July 13, 2020, an online questionnaire was sent to dentists using Google Forms.

Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats.

Objective: The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats.

Background: Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research.

Methods: Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar.

Kinesio taping use to reduce pain and edema after third molar extraction surgery: A randomized controlled split-mouth study.

Objective: Evaluating Kinesio Taping (KT) use to reduce pain and edema in individuals subjected to surgical extraction of impacted mandibular third molars.

Materials And Methods: Thirteen individuals (5 men and 8 women, mean age: 23.25 years) were subjected to mutual extraction of two mandibular third molars based on the same surgical protocol and pharmacological approach.

Digital smile design for gummy smile correction.

Some tools can be used as an aid to the surgical planning for gummy smile correction, such as digital smile design (DSD), which allows to determine patterns of harmony and eventual asymmetries between teeth and gums. This work aims to report a gummy smile correction using DSD as reverse planning. The clinical examination revealed the presence of gummy smile and extensive bone exostosis in the premolar region.

Probiotic Administration Increases Mandibular Bone Mineral Density on Rats Exposed to Cigarette Smoke Inhalation.

Smoking is one of the main risk factor for periodontal disease, increasing its prevalence and severity. Probiotics emerged as an alternative for the prevention and treatment of many diseases, since it can modulate the host immune response and modify the modify the microbiome. Therefore, the aim of the present study is to evaluate the effects of probiotic administration on the periodontal tissues in rats exposed to cigarette smoke inhalation (CSI).

Effects of low level laser therapy in cancer cells-a systematic review of the literature.

The aim of the present study was to conduct a systematic review regarding the use of photobiomodulation (PBM) therapy in tumoral cells, addressing the different types of lasers and parameters used. An electronic search was performed in PubMed/MEDLINE, Cochrane Library, and EMBASE databases. The inclusion criteria were being an in vitro study, with tumoral cells, and have at least one group of treatment with low-power laser and a control group (without treatment).

Effects of the prebiotic mannan oligosaccharide on the experimental periodontitis in rats.

Aim: To evaluate the effect of the prebiotic (PREB) mannan oligosaccharide (MOS) on the progression of the experimental periodontitis (EP) and intestinal morphology in rats.

Materials And Methods: Forty rats were randomly allocated into groups (n = 10): C (control), PREB, EP and EP-PREB. Groups PREB and EP-PREB received MOS incorporated into the feed daily.

Probiotic therapy reduces inflammation and improves intestinal morphology in rats with induced oral mucositis.

The aim of the present study was to evaluate the effect of systemic administration of probiotics (PROB) on the progression of experimentally induced oral and intestinal mucositis in rats immunosuppressed by chemotherapy (5-fluorouracil: 5-FU). Twenty-four rats were divided into the following groups (n=6): GC (control), GPROB, G5FU and G5-FU/PROB. Groups GPROB and G5-FU/PROB received 1 g of probiotic incorporated into each 100 g of feed (Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacilllus acidophilus), beginning 30 days before oral mucositis induction.

Comparison of Two Screw-Retained Free Gingival Grafting Techniques.

Free gingival graft is a predictable technique for increasing the amount of attached gingiva and root coverage; however, its use is limited for cosmetic reasons. To overcome this issue, this study sought to compare 2 free gingival graft techniques that use oral screws to attach grafts. Free gingival graft was performed on teeth 44 to 46 using the traditional technique, while on the opposite side, on teeth 34 to 36, partly epithelialized free gingival grafts were performed.

LLLT actives MMP-2 and increases muscle mechanical resistance after nerve sciatic rat regeneration.

The purpose of this study was to analyze the low-level laser therapy (LLLT) on metalloproteinase expression and the mechanical strength of skeletal muscle regeneration after peripheral nerve injury. Rats were subjected to crush injury of the right sciatic nerve, followed by LLLT (830 nm, 35, 70, 140, and 280 J/cm) for 21 consecutive days. Functional gait analysis was performed at weekly intervals and the animals were sacrificed after the last evaluation at day 21 for collection of the gastrocnemius muscles, which were submitted to analysis of resistance, and the tibialis anterior, for evaluation of metalloproteinase-2 (MMP-2).

Socket Preservation Therapy with Acellular Dermal Matrix and Mineralized Bone Allograft After Tooth Extraction in Humans: A Clinical and Histomorphometric Study.

The aim of this study was to analyze through clinical and histomorphometric parameters the use of acellular dermal matrix (ADM) with or without mineralized bone allograft (AB) on bone formation in human alveoli after a 6- to 8-month healing period. A total of 19 patients in need of extraction of the maxillary anterior teeth were selected and randomly assigned to the test group (ADM plus AB) or to the control group (ADM only). Clinical and histomorphometric measurements and histologic analysis were recorded 6 to 8 months after ridge preservation procedures.

A Randomized Comparative Study of Two Techniques to Optimize the Root Coverage Using a Porcine Collagen Matrix.

The aim of this randomized controlled clinical study was to compare the extended flap technique (EFT) with the coronally advanced flap technique (CAF) using a porcine collagen matrix (PCM) for root coverage. Twenty patients with two bilateral gingival recessions, Miller class I or II on non-molar teeth were treated with CAF+PCM (control group) or EFT+PCM (test group). Clinical measurements of probing pocket depth (PPD), clinical attachment level (CAL), recession height (RH), keratinized tissue height (KTH), keratinized mucosa thickness (KMT) were determined at baseline, 3 and 6 months post-surgery.

Comparative study of two surgical techniques for root coverage of large recessions in heavy smokers.

Reduced root coverage due to diminished periodontal vascularity can be expected in heavy smokers. The aim of this study was to evaluate the root coverage obtained for large gingival recessions in heavy smokers using two different surgical techniques. Twenty heavy smokers were selected.

Influence of periodontal tissue thickness on buccal plate remodelling on immediate implants with xenograft.

Aim: To evaluate the influence of gingival thickness and bone grafting on buccal bone plate remodelling after immediate implant placement in sockets with thin buccal bone, using a flapless approach.

Materials And Methods: The gingiva of eight dogs was thinned at one side of the mandible, mandibular premolars were extracted without flaps, and four implants were installed on each side at 1.5 mm from the buccal bone.

Palatal harvesting technique modification for better control of the connective tissue graft dimensions.

Subepithelial connective tissue graft (SCTG) has been extensively used for a variety of clinical applications. However, the surgical procedure may not allow control of graft thickness. The purpose of this case series is to illustrate a modification to the single incision palatal harvesting technique in order to control the SCTG thickness without increasing patient discomfort.

Influence of periodontal biotype on buccal bone remodeling after tooth extraction using the flapless approach with a xenograft: a histomorphometric and fluorescence study in small dogs.

Background: Several approaches have been used to counteract alveolar bone resorption after tooth extraction.

Purpose: The aim of the present study was to evaluate the influence of gingival thickness and bone grafting on buccal bone remodeling in extraction sockets with thin buccal bone, using a flapless approach.

Materials And Methods: The gingiva of 8 dogs was thinned at one side of the mandible and mandibular premolars were extracted without flaps.

The influence of the periodontal biotype on peri-implant tissues around immediate implants with and without xenografts. Clinical and micro-computerized tomographic study in small Beagle dogs.

Objective: Soft tissues and buccal bone plate remodeling after immediate implantation in sockets with thin buccal bone, using the flapless approach with or without bone graft into the buccal gap, was compared between sites with thin and normal gingiva.

Material And Methods: Eight dogs had the gingiva of one side of the mandible thinned, the mandibular premolars were extracted without flaps, and 4 implants were installed in each side, positioned 1.5 mm from the buccal bone.

Morphological, functional and biochemical characterization of canine gingival fibroblasts.

As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content.

Treatment of gingival recessions in heavy smokers using two surgical techniques: a controlled clinical trial.

Smokers have small root coverage which is associated with bad vascularity of periodontal tissues. This study evaluated a technique that can increase the blood supply to the periodontal tissues compared with a traditional technique. Twenty heavy smokers (10 males and 10 females) with two bilateral Miller class I gingival recessions received coronally positioned flaps in one side (Control group)and extended flap technique in the other side (Test group).