Identification of R-Spondin Gene Signature Predictive of Metastatic Progression in BRAFV-Positive Papillary Thyroid Cancer.

Papillary thyroid carcinoma (PTC) is the most common malignancy of the thyroid gland and early stages are curable. However, a subset of PTCs shows an unusually aggressive phenotype with extensive lymph node metastasis and higher incidence of locoregional recurrence. In this study, we investigated a large cohort of PTC cases with an unusual aggressive phenotype using a high-throughput RNA sequencing (RNA-Seq) to identify differentially regulated genes associated with metastatic PTC.

NDRG1 deficiency is associated with regional metastasis in oral cancer by inducing epithelial-mesenchymal transition.

Regional metastasis is the single most important prognostic factor in oral squamous cell carcinoma (OSCC). Abnormal expression of N-myc downstream-regulated genes (NDRGs) has been identified to occur in several tumor types and to predict poor prognosis. In OSCC, the clinical significance of deregulated NDRG expression has not been fully established.

In vitro antimicrobial and anticancer properties of TiO blow-spun nanofibers containing silver nanoparticles.

Nanosilver immobilized on TiO nanometric fibers (Ag/TiO) was produced by solution blow spinning and characterized using scanning electron microscopy, transmission electron microscopy, N adsorption/desorption, X-ray diffraction, X-ray photoelectron spectroscopy, water contact angle, and inductively coupled plasma emission spectroscopy analyses. The in vitro antimicrobial and anticancer activities of the produced nanofibers was also investigated. Ag/TiO nanofibers revealed a crystalline structure compatible with the rutile crystalline phase, as well as a mesoporous and superhydrophilic nature.

Stability of Reference Genes during Tri-Lineage Differentiation of Human Adipose-Derived Stromal Cells.

Quantitative real-time PCR can detect variations in gene expression. The identification of the stable reference genes (RGs) is necessary to evaluate the expression of specific genes of interest under various conditions in many cell types, including human adipose-derived stromal cells (hASCs). In this study, we used the algorithms BestKeeper, NormFinder, geNorm, and RefFinder to investigate the stability of 15 potential RGs (B2M, eEF1A1, GAPDH, H2AFZ, HMBS, HPRT1, PGK1, PPIA, RPL5, SDHA, TBP, TKT, TRFC, TUBB, and UBC) in hASCs during control, adipo-, chondro-, and osteogenic differentiation for 28 days.