Bactericidal activity of gold and silver nanoparticles in solution and supported on polyhihydroxybutyrate nanospheres.

This work presents the effect of Polyhydroxybutyrate nanospheres (PHB-NSs) on the bacterial activity of plasmonic nanoparticles (NPs). The PHB-NSs were used as a substrate for the metal-NPs. Silver and gold NPs in colloidal solution were synthesized by chemical reduction, while PHB-NSs were synthesized by a physical method.

Bioinformatic and functional analysis of a PHB polymerase (PhbC) from Azospirillum baldaniorum.

Background: Azospirillum baldaniorum Sp245 produces poly-β-hydroxybutyrate, a biodegradable polymer with characteristics similar to synthetic thermoplastics, including polypropylene. In the synthesis pathway, the poly-β-hydroxybutyrate synthase enzyme uses thioesters of 3-hydroxy butyryl-CoA as a substrate and catalyzes their polymerization with HS-CoA release.

Methods: A study was conducted using in silico analysis of the two phbC genes of A.

Computational Analysis of the Tripartite Interaction of Phasins (PhaP4 and 5)-Sigma Factor (σ)-DNA of Sp7.

Sp7 produces PHB, which is covered by granule-associated proteins (GAPs). Phasins are the main GAPs. Previous studies have shown phasins can regulate PHB synthesis.

Polyhydroxybutyrate Metabolism in and Its Applications, a Review.

Gram-negative accumulates approximately 80% of polyhydroxybutyrate (PHB) as dry cell weight. For this reason, this bacterium has been characterized as one of the main microorganisms that produce PHB. PHB is synthesized inside bacteria by the polymerization of 3-hydroxybutyrate monomers.

Computational Analysis of the Ligand-Binding Sites of the Molecular Chaperone OppA from .

The function of chaperones is to correct or degrade misfolded proteins inside the cell. Classic molecular chaperones such as GroEL and DnaK have not been found in the periplasm of . Some periplasmic substrate-binding proteins could be bifunctional, such as OppA.

Identification of residues for chaperone-like activity of OppA protein in Yersinia pseudotuberculosis.

Periplasmic oligopeptide binding protein (OppA) is part of a multimeric cytoplasmic membrane protein complex, whose function is known as peptide transporters found in Gram-negative bacteria. A chaperone-like activity has been found for the OppA from Yersinia pseudotuberculosis, using biochemical experiments. Through computational analysis, we selected two amino acid residues (R41 and D42) that probably are involved in the chaperone-like activity.

Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7.

Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that code for proteins with a Phasin_2 domain.

The ipdC, hisC1 and hisC2 genes involved in indole-3-acetic production used as alternative phylogenetic markers in Azospirillum brasilense.

Plant growth-promoting bacteria of the genus Azospirillum are present in the rhizosphere and as endophytes of many crops. In this research we studied 40 Azospirillum strains isolated from different plants and geographic regions. They were first characterized by 16S rDNA restriction analysis, and their phylogenetic position was established by sequencing the genes 16S rDNA, ipdC, hisC1, and hisC2.

Identification and characterization of an iron ABC transporter operon in Gluconacetobacter diazotrophicus Pal 5.

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane. We have cloned and sequenced the genes coding for the components of the iron ABC-type acquisition system of G. diazotrophicus.

Indole-3-butyric acid (IBA) production in culture medium by wild strain Azospirillum brasilense.

Some microorganisms found in the soil are able to produce substances which regulate plant growth. In this study, we show the presence of a substance associated with auxin activity, identified as indole-3-butyric acid (IBA), in Azospirillum brasilense UAP 154 growth medium. A.