Crystallization and initial X-ray diffraction analysis of the multi-domain blue light-activated histidine kinase LOV-HK in its illuminated state.

The pathogenic bacterium codes for a multi-domain dimeric cytoplasmic histidine kinase called LOV-HK, which is a key blue light-activated virulence factor in this microorganism. The structural basis of the light activation mechanism of this protein remains unclear. In this work, full-length LOV-HK was cloned, expressed and purified.