Lysine 27 dimethylation of Drosophila linker histone dH1 contributes to heterochromatin organization independently of H3K9 methylation.

Post-translational modifications (PTMs) of core histones are important epigenetic determinants that correlate with functional chromatin states. However, despite multiple linker histone H1s PTMs have been identified, little is known about their genomic distribution and contribution to the epigenetic regulation of chromatin. Here, we address this question in Drosophila that encodes a single somatic linker histone, dH1.

Combined bottom-up and top-down mass spectrometry analyses of the pattern of post-translational modifications of Drosophila melanogaster linker histone H1.

Linker histone H1 is a major chromatin component that binds internucleosomal DNA and mediates the folding of nucleosomes into a higher-order structure, namely the 30-nm chromatin fiber. Multiple post-translational modifications (PTMs) of core histones H2A, H2B, H3 and H4 have been identified and their important contribution to the regulation of chromatin structure and function is firmly established. In contrast, little is known about histone H1 modifications and their function.

Social interaction with a rhythmic rat enhances the circadian pattern of the motor activity and temperature of LL-induced arrhythmic rats.

Although light is the main factor that influences circadian rhythms, social interaction may also have a role on their regulation. Here, the influence of social interaction on rat circadian behavior was investigated, addressing the question of whether cohabitation would induce the appearance of a circadian rhythm in arrhythmic rats due to constant light. To this end, circadian rhythms of motor activity and body temperature of male and female LL-induced arrhythmic rats were studied before, during and after a 20-day period in which rats stayed in the same cage with a rat of the same sex but with stronger rhythm.