Coronaviruses () of bats in the northern Caucasus and south of western Siberia.

Introduction: Bats are natural reservoirs of coronaviruses (), which have caused three outbreaks of human disease SARS, MERS and COVID-19 or SARS-2 over the past decade. The purpose of the work is to study the diversity of coronaviruses among bats inhabiting the foothills and mountainous areas of the Republics of Dagestan, Altai and the Kemerovo region.

Materials And Methods: Samples of bat oral swabs and feces were tested for the presence of coronavirus RNA by reverse transcription-polymerase chain reaction (RT-PCR).

Academ Virus, a Novel Hantavirus in the Siberian Mole () from Russia.

To date, six hantavirus species have been detected in moles (family Talpidae). In this report, we describe Academ virus (ACDV), a novel hantavirus harbored by the Siberian mole () in Western Siberia. Genetic analysis of the complete S-, M-, and partial L-genomic segments showed that ACDV shared a common evolutionary origin with Bruges virus, previously identified in the European mole (), and is distantly related to other mole-borne hantaviruses.

Seewis virus: phylogeography of a shrew-borne hantavirus in Siberia, Russia.

Background: Hantaviral antigens were originally reported more than 20 years ago in tissues of the Eurasian common shrew (Sorex araneus), captured in European and Siberian Russia. The recent discovery of Seewis virus (SWSV) in this soricid species in Switzerland provided an opportunity to investigate its genetic diversity and geographic distribution in Russia.

Methods: Lung tissues from 45 Eurasian common shrews, 4 Laxmann's shrews (Sorex caecutiens), 3 Siberian large-toothed shrews (Sorex daphaenodon), 9 pygmy shrews (Sorex minutus), 28 tundra shrews (Sorex tundrensis), and 6 Siberian shrews (Crocidura sibirica), captured in 11 localities in Western and Eastern Siberia during June 2007 to September 2008, were analyzed for hantavirus RNA by reverse transcription-polymerase chain reaction.

[A comparative genetic analysis of the role of the brain dopaminergic systems in the regulation of behavioral elements in the "open field" test in DBA/2J and C57BL/6J mice].

The effects of intraperitoneal injections of sulpiride (10 mg/kg), bromocriptine (5 mg/kg), and alaptide (1 mg/kg) on the behavior of male C57BL/6J (C57BL) and DBA/2J (DBA) mice in the open-field test were studied. In this test, C57BL mice exhibited a significantly higher horizontal locomotor activity than DBA mice, whereas DBA mice moved in place substantially longer than C57BL mice. Dopaminergic agents had different effects on the open-field behavior in different mouse strains.

[Genetic recombination and steroid metabolism in Drosophila].

A double-species ecologo-genetical model, including Drosophila and yeast, has been used as a new methodological instrument for investigation of the physiological mechanism of recombination. Incubation of Drosophila females in the medium containing yeast of the strain mutant for ergosterol synthesis leads to suppression of temperature-induced crossing over. The mass-spectrum analysis of steroid fraction from Drosophila females has shown that incubation of the yeast medium without ergosterol results in arrest of ecdysterone synthesis.

[The effect of sterol metabolism in a model ecological system Drosophila-yeasts on the crossing-over in Drosophila].

The absence of sterols available for metabolism causes the death of Drosophila larva. Addition of suboptimal cholesterol doses to this medium allows the portion of larvae to survive. Sterol-deficient diet at the preimaginal stages leads to suppression of both spontaneous and high-temperature induced crossingover in Drosophila females.

[Effect on the fertility of female Drosophila of sterol metabolism in an ecological-genetic yeast-Drosophila system].

The consequences of sterol deficiency in feeding of adult Drosophila females have been studied. Feeding of Drosophila on nys 1 mutant strain yeast leads to significant increase of non-developed eggs in Drosophila females. The effect of sterol deficiency on oogenesis in virgin and fertilized females has been estimated using different regimens of feeding.

[Effect of sterol metabolism in the yeast-Drosophila system on the frequency of radiation-induced aneuploidy in Drosophila melanogaster oocytes].

The influence of sterol metabolism upon mutagenesis in Drosophila melanogaster was investigated using ecological-genetic yeast - drosophila system. Sterol deficiency in the organism of Drosophila was caused by using the strain of Saccharomyces cerevisiae 9-2P712 with a mutation in the nysr1 locus which blocks synthesis of ergosterol as a nutrition substrate for flies. It was concluded that maintenance of females on the mutant yeast strain causes an increase of radiation-induced X-chromosome loss in mature oocytes.