Slow Release of GA Hormone from Polymer Coating Overcomes Seed Dormancy and Improves Germination.

Seed dormancy often hinders direct seeding efforts that are attempting to restore degraded landscapes. Gibberellic acid (GA) can be applied to physiologically dormant seeds to induce germination, but this hormone is rarely effective, as it can degrade or be leached from the seed. We tested different polymer matrixes (polylactic acid, polyvinylpyrrolidone, and ethylcellulose) to apply and slowly release GA to the seed.

Inconsistency in perspective-taking during comprehension.

In order to successfully comprehend referring expressions, a listener must often consider how the speaker's perspective differs from their own. Such consideration of others' perspective is effortful and not always employed. Previous studies disagree about whether executive function predicts perspective-taking use in language comprehension.

[Working conditions of dermatologists in Brittany (France) and demographic projection: A cross-sectional survey].

Background: The number of dermatologists in Brittany is currently decreasing (-4.5% from 2010 to 2014) while waiting times for medical appointments are increasing. Our objective was to evaluate the practice activities of dermatologists and to establish a 5-year demographic projection.

Do restrained eaters restrict their caloric intake prior to drinking alcohol?

This research used multilevel modeling to investigate the hypothesis that restrained eaters increase dietary restriction when they expect to drink alcohol. Undergraduate women (39 restrained eaters; 40 unrestrained eaters) monitored their food and alcohol intake for ten days. Restrained eaters, on average, consumed fewer calories per day than unrestrained eaters.

Psychometric properties of measures of eating disorder risk in Latina college women.

Objective: Latina college women are at risk for eating disorders (EDs), but assessment is problematic, because the psychometric properties of risk factor measures have not been evaluated with this group.

Method: Reliability and validity estimates of the Eating Disorder Examination Questionnaire, Body Esteem Scale, Sociocultural Attitudes Toward Appearance Questionnaire-3, and Body Shape Questionnaire were examined in a sample of 173 female Latina college students.

Results: Cronbach α coefficients (.

Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development.

Different molecular pathways involved in maintaining mitochondrial function are of fundamental importance to control cellular homeostasis. Mitochondrial i-AAA protease is part of such a surveillance system and PaIAP is the putative ortholog in the fungal aging model Podospora anserina. Here we investigated the role of PaIAP in aging and development.

Mitochondrial protein quality control systems in aging and disease.

Preserving the integrity of proteins, biomolecules prone to molecular damage, is a fundamental function of all biological systems. Impairments in protein quality control (PQC) may lead to degenerative processes, such as aging and various disorders and diseases. Fortunately, cells contain a hierarchical system of pathways coping protein damage.

Mitochondrial pathways governing stress resistance, life, and death in the fungal aging model Podospora anserina.

Work from more than 50 years of research has unraveled a number of molecular pathways that are involved in controlling aging of the fungal model system Podospora anserina. Early research revealed that wild-type strain aging is linked to gross reorganization of the mitochondrial DNA. Later it was shown that aging of P.

Detection of native-state nonadditivity in double mutant cycles via hydrogen exchange.

Proteins have evolved to exploit long-range structural and dynamic effects as a means of regulating function. Understanding communication between sites in proteins is therefore vital to our comprehension of such phenomena as allostery, catalysis, and ligand binding/ejection. Double mutant cycle analysis has long been used to determine the existence of communication between pairs of sites, proximal or distal, in proteins.

Increasing mitochondrial superoxide dismutase abundance leads to impairments in protein quality control and ROS scavenging systems and to lifespan shortening.

The fungal aging model Podospora anserina contains three superoxide dismutases (SODs) in different cellular compartments. While PaSOD1 represents the Cu/Zn isoform located in the cytoplasm and in the mitochondrial inter-membrane space, PaSOD2 localizes to the perinuclear ER. PaSOD3, a protein with a manganese binding domain and a mitochondrial targeting sequence (MTS) is the mitochondrial SOD.

Increasing organismal healthspan by enhancing mitochondrial protein quality control.

Degradation of damaged proteins by members of the protein quality control system is of fundamental importance in maintaining cellular homeostasis. In mitochondria, organelles which both generate and are targets of reactive oxygen species (ROS), a number of membrane bound and soluble proteases are essential components of this system. Here we describe the regulation of Podospora anserina LON (PaLON) levels, an AAA(+) family serine protease localized in the matrix fraction of mitochondria.

Age-related cellular copper dynamics in the fungal ageing model Podospora anserina and in ageing human fibroblasts.

In previous investigations an impact of cellular copper homeostasis on ageing of the ascomycete Podospora anserina has been demonstrated. Here we provide new data indicating that mitochondria play a major role in this process. Determination of copper in the cytosolic fraction using total reflection X-ray fluorescence spectroscopy analysis and eGfp reporter gene studies indicate an age-related increase of cytosolic copper levels.

Medical homicide and extreme negligence.

Deaths that occur during medical care for the treatment of a disease are rarely certified as homicides. Some "medical" deaths, however, have been criminally prosecuted for manslaughter, reckless endangerment, or reckless homicide. We describe 5 deaths due to medical complications that underwent criminal prosecution.

The point prevalence of bulimic disorders from 1990 to 2004.

Objective: This study investigated the point prevalence of probable cases of bulimia nervosa (BN), eating disorder not otherwise specified (EDNOS), and specific eating disorder symptomatology among 6,844 undergraduate women at a single site, examining changes across five 3-year time periods and on a yearly basis from 1990 to 2004.

Method: Participants completed a self-report checklist that assessed the diagnostic criteria for BN (American Psychiatric Association, Diagnostic and Statistical Manual of Mental Disorders, 1994) and the Bulimia Test (Smith and Thelen, J Consult Clin Psychol, 52, 863-872, 1984) (BULIT) or Bulimia Test-Revised (Thelen et al., Psychol Assess, 3, 119-124, 1991) (BULIT-R).

Do adherence variables predict outcome in an online program for the prevention of eating disorders?

Unlike traditional interventions, Internet interventions allow for objective tracking and examination of the usage of program components. Student Bodies (SB), an online eating disorder (ED) prevention program, significantly reduced ED attitudes/behaviors in college-aged women with high body image concerns, and reduced the development of EDs in some higher risk subgroups. The authors investigated how adherence measures were associated with ED attitudes and behaviors after treatment.

Randomized, controlled trial of an internet-facilitated intervention for reducing binge eating and overweight in adolescents.

Objective: This study examined the efficacy of an Internet-facilitated intervention for weight maintenance and binge eating in adolescents.

Methods: A total of 105 adolescent male and female high school students at risk for overweight (mean age: 15.1 +/- 1.

Eating Disorder Examination Questionnaire (EDE-Q): norms for undergraduate women.

Objective: This research presents normative data on the Eating Disorder Examination Questionnaire (Fairburn and Beglin, Int J Eat Disorder, 16, 363-370, 1994) (EDE-Q) for samples of undergraduate women in the United States.

Method: College women (N = 723), ages 18-25, completed the EDE-Q as part of a larger assessment battery.

Results: Average scores, standard deviations, and percentile ranks for the raw Restraint, Eating Concern, Shape Concern, and Weight Concern subscales and the Global score are reported.

The clinical significance of loss of control over eating in overweight adolescents.

Objective: Given limited data on the diagnostic validity of binge eating disorder (BED) in adolescents, this study sought to characterize overweight adolescents according to types of overeating episodes.

Method: Ninety-six adolescents (ages 13-17 years) with recurrent binge eating (BE), loss of control with or without overeating (subclinical BE; SUB), overeating without loss of control (OE), and no overeating or loss of control episodes (CONTROL) were compared on weight/shape concerns and depressive symptoms using ANCOVA and post-hoc least squares difference tests.

Results: BE and SUB adolescents had higher weight/shape concern scores than OEs and CONTROLs (ps < .

Myeloid translocation gene family members associate with T-cell factors (TCFs) and influence TCF-dependent transcription.

Canonical Wnt signaling is mediated by a molecular "switch" that regulates the transcriptional properties of the T-cell factor (TCF) family of DNA-binding proteins. Members of the myeloid translocation gene (MTG) family of transcriptional corepressors are frequently disrupted by chromosomal translocations in acute myeloid leukemia, whereas MTG16 may be inactivated in up to 40% of breast cancer and MTG8 is a candidate cancer gene in colorectal carcinoma. Genetic studies imply that this corepressor family may function in stem cells.

Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.

The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle. Previous studies have demonstrated that, in response to ionizing radiation, Cdc6 is ubiquitinated by the anaphase promoting complex (APC(Cdh1)) in a p53-dependent manner. We find, however, that DNA damage caused by UV irradiation or DNA alkylation by methyl methane sulfonate (MMS) induces Cdc6 degradation independently of p53.

Eating disorders and alcohol use: group differences in consumption rates and drinking motives.

Alcohol use and drinking motives were investigated among college women divided into four probable eating disorder groups: Bulimia Nervosa, purging subtype (BN n=16) Binge Eating Disorder (BED n=30) Eating Disorder, Not Otherwise Specified (EDNOS n=85) and Non-Eating Disordered Controls (NEDC n=252). Participants completed questionnaires that assessed eating behaviors and attitudes, motives for drinking alcohol, quantity and frequency of alcohol use, and binge drinking. The BED group reported greater weekend alcohol consumption and binge drinking than the EDNOS and NEDC groups.

Distinct action of the retinoblastoma pathway on the DNA replication machinery defines specific roles for cyclin-dependent kinase complexes in prereplication complex assembly and S-phase progression.

The retinoblastoma (RB) and p16ink4a tumor suppressors are believed to function in a linear pathway that is functionally inactivated in a large fraction of human cancers. Recent studies have shown that RB plays a critical role in regulating S phase as a means for suppressing aberrant proliferation and controlling genome stability. Here, we demonstrate a novel role for p16ink4a in replication control that is distinct from that of RB.

Prevention of eating disorders in at-risk college-age women.

Context: Eating disorders, an important health problem among college-age women, may be preventable, given that modifiable risk factors for eating disorders have been identified and interventions have been evaluated to reduce these risk factors.

Objective: To determine if an Internet-based psychosocial intervention can prevent the onset of eating disorders (EDs) in young women at risk for developing EDs.

Setting: San Diego and the San Francisco Bay Area in California.