Correction: Boia-Ferreira et al. TCTP from (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism. 2019, , 1489.

In the original publication [...

Bloodstream Isolates: Investigation of Clonal Relationship, Resistance Profile, Virulence and Biofilm Formation.

has been recognized as a relevant opportunistic pathogen, particularly its persistence in neonatal ICUs around the world. Therefore, the aim of this study was to describe the epidemiological profile of clinical isolates of and to characterize the factors involved in the persistence and pathogenesis of these strains isolated from blood cultures collected in a hospital in the interior of the state of São Paulo, Brazil. A total of 141 strains were submitted to detection of the gene and S typing by multiplex PCR.

Molecular Epidemiology of and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities.

At present, multidrug-resistant microorganisms are already responsible for community-acquired infections. Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious public health risk worldwide because of the rapid spread and diversification of pandemic clones that are characterized by increasing virulence and antimicrobial resistance. The aim of this study was to identify the prevalence and factors associated with nasal, oral and rectal carriage of S.

Efficient tyrosinase nano-inhibitor based on carbon dots behaving as a gathering of hydrophobic cores and key chemical group.

Small organic molecules have been extensively applied to achieve enzymatic inhibition. Although numerous efforts have been made to deliver efficient inhibitors, small inhibitors applications are hindered by many drawbacks. Moreover, reporters comprising nanoparticle inhibitory activity against enzymes are very scarce in the literature.

Evaluation of toxicity and mutagenicity of a synthetic effluent containing azo dye after Advanced Oxidation Process treatment.

Approximately 20% industrial water pollution comes from textile dyeing process, with Azo dyes being a major problem in this scenario and requiring new forms of efficient treatment. Effluent treatments using the Advanced Oxidation Processes (AOP) are justified by the potential of application in the dyed effluent treatments once they can change the Azo dye chemical structure. Thus, this study aimed to evaluate the toxicity and mutagenic capacity of a synthetic effluent containing Amido Black 10B (AB10B) azo dye before treatment with AOP, named Gross Synthetic Effluent (GSE), and after the AOP, named Treated Synthetic Effluent (TSE).

Asymptomatic Leishmania infection in blood donors from a major blood bank in Northeastern Brazil: a cross-sectional study.

This study has estimated the risk of Leishmania transmission via blood transfusion in one of the largest blood banks in Northeastern Brazil, where visceral leishmaniasis is endemic. Five hundred blood samples from donors were tested for circulating Leishmania spp. DNA by real-time PCR.

Development and validation of an analytical method for the extraction, identification, and quantification of multi-mycotoxins in beer using a modified QuEChERS procedure and UHPLC-MS/MS.

Although beer is one of the most popular alcoholic beverages in the world, there is no specific legislation regarding contaminants, especially mycotoxins, for this product. The present manuscript reports the development and validation of an analytical methodology based on the QuEChERS approach, followed by quantification via UHPLC-MS/MS for the simultaneous determination of seventeen mycotoxins in beer. During the validation, amatrix effect was observed for 82% of the analytes.

Methodology development based on "dilute and shoot" and QuEChERS for determination of multiple mycotoxins in cocoa by LC-MS/MS.

This work proposes an extraction method based on the "dilute and shoot" approach and QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) for the simultaneous determination of 42 mycotoxins (34 quantified and 8 qualitatively studied) in dried cocoa bean samples. The purpose of the developed methodology was the reduction of co-extractives from the matrix and an efficient extraction without a cleanup step, and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). In order to obtain the best extraction conditions, gravimetric tests were performed and parameters that influenced the extraction efficiency were evaluated, such as the proportion of extraction phases, amount of salt, acidification, and extraction time.

TCTP from (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism.

LiTCTP is a toxin from the Translationally Controlled Tumor Protein (TCTP) family identified in brown spider venoms. These proteins are known as histamine-releasing factors (HRF). TCTPs participate in allergic and anaphylactic reactions, which suggest their potential role as therapeutic targets.

Furan inhibitory activity against tyrosinase and impact on B16F10 cell toxicity.

Many skin disorders and diseases are related to tyrosinase activity, in particular, due to the vital role played by this enzyme in the melanogenic process. Although numerous natural and synthetic tyrosinase inhibitors have been published, substantial efforts have been made to understand the influence of tyrosinase inhibition on the viability of melanoma cells. Here, we assess the impact of two keto-derivatives: 2-acetyl-furan (F1), furfural-acetone (F2), and two carboxyl-derivatives: 2-furan-acrylic acid (F3), 5-methyl-2-furan-acrylic acid (F4), on the mushroom tyrosinase (mTYR) activity, by applying spectroscopic, kinetic and theoretical techniques.

Development and validation of a multianalyte method for quantification of mycotoxins and pesticides in rice using a simple dilute and shoot procedure and UHPLC-MS/MS.

In the present manuscript an analytical methodology for the simultaneous determination of ten mycotoxins and six pesticides in rice was developed. This methodology comprises the application of the dilute and shoot protocol followed by quantification via UHPLC-MS/MS. The methodology was validated and all figures of merit shown to be within the limits established by regulation.

Evaluation of two novel leptospiral proteins for their interaction with human host components.

Pathogenic species of the genus Leptospira are the etiological agents of leptospirosis, the most widespread zoonosis. Mechanisms involved in leptospiral pathogenesis are not well understood. By data mining the genome sequences of Leptospira interrogans we have identified two proteins predicted to be surface exposed, LIC10821 and LIC10064.

Leptospira spp.: Novel insights into host-pathogen interactions.

Leptospirosis is a widespread zoonosis caused by pathogenic Leptospira spp. It is an important infectious disease that affects humans and animals. The disease causes economic losses as it affects livestock, with decreased milk production and death.