Publications by authors named "Lucas Fedalto"
The use of biological components in the development of new methods of analysis and point-of-care (POC) devices is an ever-expanding theme in analytical chemistry research, due to the immense potential for early diagnosis of diseases and monitoring of biomarkers. In the present work, the evaluation of an electrochemical microfluidic device based on the immobilization of horseradish peroxidase (HRP) enzyme into chemically treated cotton threads is described. This bioreactor was used as a channel for the build of the microfluidic device, which has allowed to use of a non-modified screen-printed electrode (SPE) as an amperometric detector.
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- The development of a low-cost microfluidic system enables green and solvent-free chromatographic separations using cotton threads for efficient electrochemical detection.
- The system relies on a simple assembly with ion exchange mechanisms and gold electrodes built directly onto the cotton, eliminating the need for mechanical pumps.
- Effective separation of ascorbic acid and dopamine is achieved with low detection limits, minimal waste generation, and affordability, making it suitable for analyzing biological samples without prior treatment.