Ovarian tissue vitrification: the use of a novel metal closed system for clinical grade cryopreservation.

Objective: To investigate whether a metal chamber is an appropriate system for vitrification of ovarian tissue under clinical grade.

Methods: Experimental study, control versus treatment. Bovine ovarian cortices cut in 1x1x1 mm fragments were vitrified using ethylene glycol and dimethyl sulfoxide inside steel cryovials, whose bases were in touch with Liquid Nitrogen (LN2).