Simulation of a new neutron calibration laboratory in Brazil using MCNP5.

Metrobras, a private company in Brazil, is developing a new laboratory for the calibration of neutron detectors in collaboration with the National Laboratory for Metrology of Ionizing Radiation (LNMRI). This work aimed at assessing neutron spectra, ambient dose equivalent rates, and neutron scattering in the calibration room of Metrobras Neutron Detectors Calibration Laboratory (LCDNM) using Monte Carlo simulation with MCNP5. Three models were simulated: vacuum, air, and the complete with the several materials employed in the actual room.

Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.

Background: The Bacillus subtilis endo-β-1,4-glucanase (BsCel5A) hydrolyzes β-1,3-1,4-linked glucan, and the enzyme includes a family 3 carbohydrate-binding module (CBM3) that binds β-1,4-linked glucan.

Methods: Here we investigate the BsCel5A β-1,3-1,4 glucanase activity after exchanging the CBM3 domain for the family 11 CBM from Ruminiclostridium thermocellum celH (RtCBM11) having β-1,3-1,4 glucan affinity.

Results: The BsCel5A-RtCBM11 presents a 50.