Publications by authors named "Lucas D Lopes"

Understanding how microbial communities are shaped across spatial dimensions is of fundamental importance in microbial ecology. However, most studies on soil biogeography have focused on the topsoil microbiome, while the factors driving the subsoil microbiome distribution are largely unknown. Here we used 16S rRNA amplicon sequencing to analyse the factors underlying the bacterial β-diversity along vertical (0-240 cm of soil depth) and horizontal spatial dimensions (~500,000 km ) in the U.

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Root exudates shape the rhizosphere microbiome, but little is known about the specific compounds in root exudates that are important. Here, we investigated the impacts of the plant-synthesized phytohormones indole-3-acetic acid (IAA) and abscisic acid (ABA) exuded by roots on the maize rhizobacterial communities. To identify maize genotypes that differed in the root exudate concentrations of IAA and ABA, we screened hundreds of inbred lines using a semi-hydroponic system.

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Root exudates contribute to shaping the root-associated microbiomes, but it is unclear which of the many exudate compounds are important in this process. Here, we focused on understanding the influence of sugars and jasmonic acid (JA) concentrations in maize root exudates on the rhizobacterial communities. Twelve maize genotypes were identified with variable concentrations of sugars and JA based on a screening of 240 maize genotypes grown in a semihydroponic system.

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Root exudates are important for shaping root-associated microbiomes. However, studies on a wider range of metabolites in exudates are required for a comprehensive understanding about their influence on microbial communities. We identified maize inbred lines that differ in exudate concentrations of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and γ-aminobutyric acid (GABA) using a semi-hydroponic system.

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Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Hadidi across desert populations.

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Article Synopsis
  • Belowground microbiomes play a key role in supporting plant growth by aiding nutrient cycling and mitigating stresses related to nitrogen limitations.
  • An analysis was conducted on the bacterial communities associated with different sweet sorghum genotypes under low and high nitrogen conditions to assess their sensitivity to nitrogen stress.
  • Results showed distinct bacterial communities in nitrogen-stress tolerant and sensitive genotypes only under high nitrogen, and certain beneficial bacteria were more abundant in tolerant plants under low nitrogen, suggesting the importance of these microbial interactions in stress management.
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Soil pH is a major factor shaping bulk soil microbial communities. However, it is unclear whether the belowground microbial habitats shaped by plants (e.g.

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Article Synopsis
  • - This study explored how the abundance, composition, and diversity of microbial communities vary at different soil depths in corn and soybean fields in Iowa using DNA sequencing techniques.
  • - Findings showed that microbial richness and diversity decrease with increasing soil depth, while some microbial phyla became more dominant in deeper soils beyond 90 cm.
  • - Soil depth was identified as the key factor influencing microbial community structure, alongside soil properties like organic matter and water saturation, highlighting the importance of considering deeper soils in agricultural practices for improved sustainability and ecosystem health.
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  • Pseudomonas putida is a diverse group of bacteria with various species involved in important functions like promoting plant growth and cleaning up environmental pollutants.* -
  • The study compared 19 P. putida isolates from sugarcane rhizosphere and bulk soil to assess their genomic and metabolic differences, revealing that these two populations cluster into distinct groups based on phylogenomics.* -
  • Comparative analyses highlighted significant differences in genetic functions and metabolic profiles between rhizosphere and bulk soil strains, including unique pathways for D-galactonic acid and cellulose, which may influence their adaptation to different soil environments.*
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  • A species of Gram-positive bacteria, known for producing corynetoxin, causes a fatal condition called annual ryegrass toxicity in grazing animals and has shown low genetic diversity over 30 years of sampling in Australia.
  • Phylogenomic analysis revealed that the bacteria form nine distinct species groups, with one species displaying a significantly reduced genome and minimal genetic diversity, which may have resulted from a key evolutionary event.
  • The unique features of this species include the presence of a CRISPR system, which helps it defend against bacteriophages, and the findings suggest a three-stage process that highlights the role of bacteriophage interactions in shaping the bacteria's genome and genetic characteristics.
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Article Synopsis
  • The study investigates how different soil environments (bulk soil vs. rhizosphere) affect the microbial communities, particularly focusing on the Pseudomonas koreensis subgroup.
  • Researchers sequenced the genomes of 53 bacterial isolates from both environments and observed distinct genomic differences between them, suggesting a separation into different ecotypes.
  • Notable changes included increased phosphatases in rhizosphere isolates and xylose-utilizing genes in bulk soil isolates, indicating that specific genes can influence growth capacity depending on the habitat.
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Secondary metabolites are synthesized by many microorganisms and provide a fitness benefit in the presence of competitors and predators. Secondary metabolism also can be costly, as it shunts energy and intermediates from primary metabolism. In spp.

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Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp.

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Fluorescent Pseudomonas spp. are widely studied for their beneficial activities to plants. To explore the genetic diversity of Pseudomonas spp.

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The rhizosphere harbors one of the most complex, diverse, and active plant-associated microbial communities. This community can be recruited by the plant host to either supply it with nutrients or to help in the survival under stressful conditions. Although selection for the rhizosphere community is evident, the specific bacterial traits that make them able to colonize this environment are still poorly understood.

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The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal's digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE).

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